Mitochondrion Mitochondria Structural Features The inner membrane is protein rich (4:1 protein:lipid).not permeable Inner > Membrane and contains within it the Outer electron transporter,ATP Membrane synthase,membrane transport proteins. NThe outer membrane is readily permeable to molecules with Cristae molecular weights below 10,000 Da. Matrix Figure 1
Mitochondrion The inner membrane is protein rich (4:1 protein:lipid). not permeable and contains within it the electron transporter, ATP synthase, membrane transport proteins. The outer membrane is readily permeable to molecules with molecular weights below 10,000 Da.
ADP+Pi ATP 2H→Respiratory→O2→H2O chain AERL-COA acid cyele NA NEinaecdoityrial meaneks -NADH Transfer of reducing equavalents to oxygen occurs in inner mitochondrial membrane
2H Respiratory chain O2 H2O ADP+Pi ATP Transfer of reducing equavalents to oxygen occurs in inner mitochondrial membrane
1.Respiratory chain NADH 。2H Comple Complex Complex IV >Definition H+and e are passed through a set of electron transporters embedded in inner mt membrane to O2.Respiratory chain comprises enzymatic series electron transporters (electron donors and acceptors),also called electron transfer chain (ETC)
➢Definition H+ and e - are passed through a set of electron transporters embedded in inner mt membrane to O2 . Respiratory chain comprises enzymatic series electron transporters (electron donors and acceptors), also called electron transfer chain（ETC）. 1. Respiratory chain
>Composition Electron transporters (2H 2H++2e) 4 protein complexes +2 free electron transporter (CoQ and Cyt c) Human ETC protein complex mw polypeptides cofactor Complex I 880 44 FMN,Fe-S (NADH-Q reductase) ComplexII 140 4 FAD,Fe-S,Cytb (succinate-Q reductase) ComplexⅢ 280 ≥11 Rieske Fe-S,CytbL/H, (Q-Cytc reductase) Cytc1 ComplexIV 200 14 Cua、Cyta、Cyta3Cug (CytC oxidase)
4 protein complexes + 2 free electron transporter（CoQ and Cyt c ） Human ETC protein complex mw polypeptides cofactor ComplexⅠ 880 44 FMN, Fe-S （NADH-Q reductase） ComplexⅡ 140 4 FAD, Fe-S, Cytb （succinate-Q reductase） ComplexⅢ 280 ≥11 Rieske Fe-S, CytbL/H, (Q-Cytc reductase） Cytc1 ComplexⅣ 200 14 CuA、Cyta、 Cyta3、CuB （CytC oxidase） ➢Composition Electron transporters（2H 2H+ + 2e）
Location of each complex of respiratory chain Cytochrome c cytosol Inner membrane matrix NADH FADH2 09 CoQ and Cytochrome c are mobile component
Location of each complex of respiratory chain cytosol matrix Cytochrome c Inner membrane CoQ and Cytochrome c are mobile component