上游充通大学 Shanghai Jiao Tong University 1、微生物培养的常用器具及其灭菌 常用的器具：试管、三角瓶、培养皿等
Shanghai Jiao Tong University 1、微生物培养的常用器具及其灭菌 常用的器具：试管、三角瓶、培养皿等
B C 图V-4棉塞 图V-3 摆斜面 A.正确；B、C不正确 A B C 图V-6通气塞 A.配制时纱布塞法；B.灭菌时包牛皮纸；C.培养的纱布翻出
Shanghai Jiao Tong University
上游充通大睾 Shanghai Jiao Tong University 2、接种操作 无菌操作：火焰附近（酒精灯、 煤气灯)进行；或在无菌箱或操 作室内无菌的环境下进行
Shanghai Jiao Tong University 2、接种操作 无菌操作：火焰附近（酒精灯、 煤气灯）进行；或在无菌箱或操 作室内无菌的环境下进行
接种操 (a)Loop is heated until red-hot and cooled in air (b)Tube is uncapped. 作 briefly. (f)The tube is recapped. Loop is reheated before (d)(Sample is removed (b) being taken out of service on sterile loop. (e)After removing (c)Tip of tube is run through sample on loop,the the flame. tube is reflamed and the sample transferred to a sterile medium. (d) (e) (f)
(a) Loop is heated until red-hot and cooled in air briefly. (b) Tube is uncapped. (c) Tip of tube is run through the flame. (d) Sample is removed on sterile loop. (e) After removing sample on loop, the tube is reflamed and the sample transferred to a sterile medium. (f) The tube is recapped. Loop is reheated before being taken out of service 接种操 作
接种至新鲜培养基平板上 FLASH (a) (b) (a)Loop is sterilized,and then a (b)Streak is made over a sterile agar loopful of inoculum is removed plate,spreading out the organisms. from tube. Following the initial streak,subsequent streaks are made at angles to it,the loop being resterilized between streaks
(b) Streak is made over a sterile agar plate, spreading out the organisms. Following the initial streak, subsequent streaks are made at angles to it, the loop being resterilized between streaks. 接种至新鲜培养基平板上 (a) Loop is sterilized, and then a loopful of inoculum is removed from tube. FLASH