RNA polymerase Sigma Sigma recognizes (core factor promoter and initiation site enzyme) Steps in RNA synthesis. 5 3 3 5' The initiation and Promoter Gene(s)to be transcribed region (light green strand) termination sites are Transcription begins; sigma released.RNA chain grows Sigma specific nucleotide 5 3 5 sequences on the DNA. 5 RNA The sigma factor allows Termination site reached;chain growth stops RNA polymerase to 5 3 3 recognize the initiation Polymerase site (the promoter).The and RNA released 5 3 sigma factor is released 5 during elongation. (a) 2012 Pearson Education.Inc
Steps in RNA synthesis. The initiation and termination sites are specific nucleotide sequences on the DNA. The sigma factor allows RNA polymerase to recognize the initiation site (the promoter). The sigma factor is released during elongation
泽充王大学 上 Shanghai Jiao Tong University 7.7.2 Where does the sentence begin?- Promoter启动子 The promoter plays a key role in the initiation of RNA synthesis. The sigma factor,as part of the RNA polymerase,is involved in promoter recognition. RNA polymerase nitiate (core enzyme) Terminate Promoter region Transcription 5 3 3 3 3 1 Gene RNA polymerase (core enzyme) Sigma aids in Sigma mRNA start recognition of 5 3 Sigma factor promoter and 1CTGTTGACAATTAATCATCGAACTAGTTAACTAGTACGCAAG CTATTCCTGTGGATAACCATGTGTATTAGAGTTAGAAAACA initiation site ATATACTCACAGCATA TCTGATCCCAGCTT 3 TTTTTGAGTTGTGTATAACCC 5TAGTTGCATGAACTCGCAT CCATAGAATGCGCGCTACT GTTCTTGACACCTTTTCGGCA CGCCCTAAAATTCGGCGTC -35 sequence Pribnow box 3 5 Consensus TTGACA TATAAT Promoter sequence e的Ihae Esemn
No. 12 Chen Feng, Shanghai Jiao Tong University 7.7.2 Where does the sentence begin? – Promoter 启动子 The promoter plays a key role in the initiation of RNA synthesis. The sigma factor, as part of the RNA polymerase, is involved in promoter recognition
Two sequences within the promoter region are high/y conserved,it is the conserved region that are recognized by sigma.启动子的高度保守区 One is about 10 bases from the start of transcription:-10 region (Pribnow box;10区（普里布诺框） The second is-35 sequence.-35 5 3 3 5 Sigma mRNA start 5 3 CTGTTGACAATTAATCATCGAACTAGTTAACTAGTACGCAAG CTATTCCTGTGGATAACCATGTGTATTAGAGTTAGAAAACA 3TGGTTCCAAAATCGCCTTTTGCTGTATATACTCACAGCATA 4. TTTTTGAGTTGTGTATAACCCCTCATTCTGATCCCAGCTT 5. TAGTTGCATGAACTCGCATGTCTCCATAGAATGCGCGCTACT 6. TTCTTGACACCTTTTCGGCATCGCCCTAAAATTCGGCGTC -35 sequence Pribnow box Consensus TTGACA TATAAT Promoter sequence FLASH 3 2012 Pearson Education,Ine
Two sequences within the promoter region are highly conserved, it is the conserved region that are recognized by sigma. 启动子的高度保守区 One is about 10 bases from the start of transcription: –10 region (Pribnow box);-10区(普里布诺框) The second is – 35 sequence. –35区 FLASH 3