小结 √噬菌体SPO1感染枯草芽孢杆菌时,其基因转录呈 时序性进行:首先是早期基因,然后是中期基因, 最后是晚期基因。 √这种转换是由噬菌体所编码的一组σ因子来控制。 这些σ因子通过与宿主的核心RNA聚合酶结合, 改变RNA聚合酶对早、中、晚期基因启动子识别 的特异性。 √宿主的σ因子对噬菌体早期基因具有特异性;而 噬菌体的g即28蛋白将这种特异性转换为针对中期 基因;噬菌体的g33和34蛋白又将特异性转换为 针对晚期基因
小结 ✓噬菌体SPO1感染枯草芽孢杆菌时,其基因转录呈 时序性进行:首先是早期基因,然后是中期基因, 最后是晚期基因。 ✓这种转换是由噬菌体所编码的一组σ因子来控制。 这些σ因子通过与宿主的核心RNA聚合酶结合, 改变RNA聚合酶对早、中、晚期基因启动子识别 的特异性。 ✓宿主的σ因子对噬菌体早期基因具有特异性;而 噬菌体的gp28蛋白将这种特异性转换为针对中期 基因;噬菌体的gp33和34蛋白又将特异性转换为 针对晚期基因
8.2 The RNA Ploymerase Encode in PhageT7 In these phases we distinguish three phases of transcription,called classes I,II, and III.(They could just as easily be called early,middle,and late,to conform to SPO1 nomenclature.) One of the five class I genes (genel)is necessary for class II and class III gene expression,The gene 1 product is a phage- specific RNA polymerase
8.2 The RNA Ploymerase Encode in PhageT7 ✓In these phases we distinguish three phases of transcription, called classes I, II, and III. (They could just as easily be called early, middle, and late, to conform to SPO1 nomenclature.) ✓One of the five class I genes (gene1) is necessary for class II and class III gene expression,The gene 1 product is a phagespecific RNA polymerase