CopyrightThe McGraw-Hill Companies,Inc.Permission required for reproduction or display. Chapter 21 DNA Replication II Detailed Mechanism Cal Harley/Geron Corporation Peter Rabinovitch,Univ.of Washington
Chapter 21 DNA ReplicationⅡ: Detailed Mechanism
2 1.1 Speed of Replication
2 1.1 Speed of Replication
measure the rate of fork movement in vitro Primosome assembly site 00 Pol IlI holoenzyme +SSB a To create a synthetic circular template for rolling circle replication,This template contained a 32p-labeled tailed. At 10 sec intervals,they removed the labeled product DNAs and measured their lengths by electrophoresis
To create a synthetic circular template for rolling circle replication，This template contained a 32p-labeled tailed. At 10 sec intervals, they removed the labeled product DNAs and measured their lengths by electrophoresis． measure the rate of fork movement in vitro
Copyright The McGraw-Hill Companies.Inc.Permission required for reproduction or display. (a) (b) (c) 1234567891011 1234567891011 他 50 2 431 9 7395 37.7 器8 16.2 162 21.5 30 162 16.2 10.8 108 20 108 108 Tailed form ll 1111I11 54 1020304050607080 Tailed Time (sec) form ll 54 -54 polⅢholoenzyme plus:(a)the preprimosomal proteins((前引发 体蛋白)(the primosomal proteins minus DnaG);or(b)DnaB alone.about 730 nt/sec
pol III holoenzyme plus: (a) the preprimosomal proteins(前引发 体蛋白) (the primosomal proteins minus DnaG); or (b) DnaB alone. about 730 nt/sec
Processivity(持续性)：the ability of the enzyme to stick to its job a long time without falling off and having to reinitiate
Processivity(持续性): the ability of the enzyme to stick to its job a long time without falling off and having to reinitiate．