18.3 The bacterial genome is a nucleoid with many supercoiled loops Average loop contains ~40 kb DNA Figure 18.6 The bacterial genome consists of a large number of loops of duplex DNA (in the form Loops secured at base by unknown of a fiber),each secured mechanism at the base to form an independent structural Loop consists of duplex DNA domain. condensed by basic proteins in unknown 清菜大兰
Figure 18.6 The bacterial genome consists of a large number of loops of duplex DNA (in the form of a fiber), each secured at the base to form an independent structural domain. 18.3 The bacterial genome is a nucleoid with many supercoiled loops
18.4 Loops,domains,and scaffolds in eukaryotic DNA MAR (matrix attachment site;also known as SAR for scaffold attachment site)is a region of DNA that attaches to the nuclear matrix. Nuclear matrix is a network of fibers surrounding and penetrating the nucleus. Scaffold of a chromosome is a proteinaceous structure in the shape of a sister chromatid pair,generated when chromosomes are depleted of histones. 清菜大当
MAR (matrix attachment site; also known as SAR for scaffold attachment site) is a region of DNA that attaches to the nuclear matrix. Nuclear matrix is a network of fibers surrounding and penetrating the nucleus. Scaffold of a chromosome is a proteinaceous structure in the shape of a sister chromatid pair, generated when chromosomes are depleted of histones. 18.4 Loops, domains, and scaffolds in eukaryotic DNA
18.4 Loops,domains, and scaffolds in eukaryotic DNA Figure 18.7 Histone- depleted chromosomes consist of a protein scaffold to which loops of DNA are anchored. Photograph kindly provided by Ulrich K. Laemmli. 清苇大当
Figure 18.7 Histonedepleted chromosomes consist of a protein scaffold to which loops of DNA are anchored. Photograph kindly provided by Ulrich K. Laemmli. 18.4 Loops, domains, and scaffolds in eukaryotic DNA
Prepare nuclei 18.4 Loops,domains,and Extract histones scaffolds in eukaryotic DNA Figure 18.8 Matrix- associated regions may be Cleave with Degrade all DNA restnction with DNAase identified by characterizing nucleases the DNA retained by the matrix isolated in vivo or C00 by identifying the fragments that can bind to MAR Add DNA the matrix from which all DNA has been removed. MAR Extract DNA and analyze 情菜大学
Figure 18.8 Matrixassociated regions may be identified by characterizing the DNA retained by the matrix isolated in vivo or by identifying the fragments that can bind to the matrix from which all DNA has been removed. 18.4 Loops, domains, and scaffolds in eukaryotic DNA