In radial immunodiffusion, Ab(e. g. horse anti human IgG)is incorporated into the gel and Ag(e.g human serum) is placed in a hole cut in the gel. ag diffuses radially out of the well into the gel and interacts with the Ab forming a ring of precipitation the diameter of which is related to the concentration of the Ag(Fig. 1) edial immunodiffusion
In radial immunodiffusion, Ab (e.g. horse antihuman IgG) is incorporated into the gel and Ag (e.g. human serum) is placed in a hole cut in the gel. Ag diffuses radially out of the well into the gel and interacts with the Ab forming a ring of precipitation, the diameter of which is related to the concentration of the Ag (Fig. 1). Radial immunodiffusion
Precipitation ring I rekicuvra ○③ Standards Fig. 1. Measurement of Ag by precipitation in gefs. Ab-containing gel is placed on a glass or plastic surface. Holes are cut in the gef and filed with Ag which diffuses radially out of the weff and interacts with the Ab in the gel Soluble complexes are initially formed but as more Ag diffuses equivalence is reached resuting in a lattice and precipitation. The diameter of the precipitation ning is related to the concentration of the Ag and, using known standards, can be quantitated and compared with the levels of Ag in oth er samples
Fig. 1. Measurement ofAg by precipitation In gels. Ab-containing gel is placed on a glass or plastic surface. Holes are cut in the gel and filled with Ag which diffuses radially out of the well and interacts with the Ab in the gel. Soluble complexes are initially formed but as more Ag diffuses equivalence is reached resulting in a lattice and precipitation. The diameter of the precipitation ring Is related to the concentration of the Ag and, using known standards, can be quantitated and compared with the levels of Ag in other samples
Similar assays have been developed in which a voltage gradient(electrophoresis)is used to speed up movement of Ag into the ab containing gel(rocket immunoelectrophoresis火箭免疫电泳)
Similar assays have been developed in which a voltage gradient (electrophoresis) is used to speed up movement of Ag into the Ab containing gel (rocket immunoelectrophoresis火箭免疫电泳)
In immunoelectrophoresis, Ags(e.g. serum)are placed in a well cut in a gel(without Ab)and electrophoresed, after which a trough is cut in the gel into which Abs(e.g. horse anti-human) are placed The abs diffuse laterally(横向扩散) to meet diffusing Ag, and lattice formation and precipitation occur permitting determination of the nature of the .gs(L'"o immunoelectrophoresis
In immunoelectrophoresis, Ags (e.g. serum) are placed in a well cut in a gel (without Ab) and electrophoresed, after which a trough is cut in the gel into which Abs (e.g. horse anti-human) are placed. The Abs diffuse laterally（横向扩散） to meet diffusing Ag, and lattice formation and precipitation occur permitting determination of the nature of the Ags (Fig. 2). immunoelectrophoresis
Fig 2 identification of antigens using gel electrophoresis Ag(Ee.g. serum)is placed in a well cut in a gel and subjected to a Antisera voltage gradient which causes the various antigens to migrate different distances through the gel dependent on their Ag charge. After electrophoresis, a trough is cut in the gel into which antibodies(e.g. horse anti-human serum) are placed. The antibodies diffuse laterally from the until they meet Ag diffusing from its location after elecphoresis of defined standards the identity of fthe ag can be determined
Fig.2 identification of antigens using gel electrophoresis. Ag(Ee.g. serum)is placed in a well cut in a gel and subjected to a voltage gradient which causes the various antigens to migrate different distances through the gel dependent on their charge. After electrophoresis, a trough is cut in the gel into which antibodies (e.g. horse anti-human serum) are placed. The antibodies diffuse laterally from the until they meet Ag diffusing from its location after elecphoresis of define3d standards, the identity of fthe Ag can be determined