Section B precipitation and agglutination 沉淀和凝集
Section B precipitation and agglutination 沉淀和凝集
Precipitation assaⅴs Combination of ab with ag results in lattice formation and precipitation if there is sufficient Ag and Ab(equivalence ). These reactions are the basis for qualitative and quantitative assays for Ag or Ab, including radial immunodiffusion(辐射免疫扩散) and immunoelectrophoresis(免疫电泳)
Combination of Ab with Ag results in lattice formation and precipitation if there is sufficient Ag and Ab (equivalence). These reactions are the basis for qualitative and quantitative assays for Ag or Ab, including radial immunodiffusion(辐射免疫扩散) and immunoelectrophoresis(免疫电泳). Key notes Precipitation assays
Agglutination assays The interaction of surface Ags on insoluble particles(e.g. cells) with specific Ab to these Ags results in agglutination of the particles Agglutination can be used to determine blood types; the presence of ab to bacteria in serum is an indication of previous or current infection; and in the Coomb's test autoantibodies to erythrocytes can d. e assaye
The interaction of surface Ags on insoluble particles (e.g. cells) with specific Ab to these Ags results in agglutination of the particles. Agglutination can be used to determine blood types; the presence of Ab to bacteria in serum is an indication of previous or current infection; and in the Coomb's test autoantibodies to erythrocytes can be assayed. Key notes Agglutination assays
1. Precipitation assays previously described. when there is both sufficient Ag and sufficient Ab. the combination of As 8 and Ab proceeds until large aggregates are formed which are insoluble in water and precipitate (equivalence). The extent to which a lattice forms depends on the relative amounts of Ag and ab present
As previously described , when there is both sufficient Ag and sufficient Ab, the combination of Ag and Ab proceeds until large aggregates are formed which are insoluble in water and precipitate (equivalence). The extent to which a lattice forms depends on the relative amounts of Ag and Ab present. 1. Precipitation assays
Lattice formation, and precipitation are the basis for several qualitative and quantitative assays for Ag or ab These assays are done in semisolid gels into which holes are cut for Ag and/or for ab and diffusion occurs until Ag and ab are at equivalence and precipitate
Lattice formation, and precipitation are the basis for several qualitative and quantitative assays for Ag or Ab. These assays are done in semisolid gels into which holes are cut for Ag and/or for Ab and diffusion occurs until Ag and Ab are at equivalence and precipitate