实验四叶绿体的分离与观察实验五植物细胞液泡系与细胞骨架的染色与观察
实验五 植物细胞液泡系与 细胞骨架的染色与观察 实验四 叶绿体的分离与观察
实验四叶绿体的分离与观察
实验四 叶绿体的分离与观察
Centrifugation can separate many types of oganellesfrom cell homogenatearmored chambersedimenting materialrotorrefrigerationvacuummotor
9.4 Isolation and Characterization of Cell Organelles (细胞器) Centrifugation can separate many types of oganelles from cell homogenate
Centrifugation can separate many types of oganellesfromcellhomogenateFractionation（细胞组分的分级分离）Centrifugation techniques:(1）Differential centrifugation（差速离心）(2）Density-gradientcentrifugation（密度梯度离心）
9.4 Isolation and Characterization of Cell Organelles (细胞器) Centrifugation can separate many types of oganelles from cell homogenate → Fractionation (细胞组分的分级分离). Centrifugation techniques: (1) Differential centrifugation (差速离心) (2) Density-gradient centrifugation (密度梯度离心)
Centrifugation can separate many types of oganellesfrom cell homogenate,Filterhomogenateto removeclumpsofunbrokencells,connectivetissue,etc.CentrifugeSupernatant(上清液)Pourout:Pourout:Pourout:600gx15,000gx100,000gx300,000gx2h10min60minPourout5minLPlasmaPelletLMitochondria,RibosomalSolubleFilteredNucleimembrane,partofhomogenatechloroplasts,subunits,(颗粒团)microsomalsmallcytoplasmlysosomes,fractionandpolyribo-(cytosol)(fragmentsofsomesperoxisomesendoplasmicreticulum)andlargepolyribosomes沉降顺序：细胞核一线粒体,溶酶体与过氧化物酶体一内质网与高尔基体一核糖体
9.4 Isolation and Characterization of Cell Organelles (细胞器) Centrifugation can separate many types of oganelles from cell homogenate, 1st Step: Differential centrifugation 沉降顺序：细胞核—线粒体,溶酶体与过氧化物酶体—内质网与高尔基体—核糖体。 Pellet (颗粒团) Supernatant (上清液)