实验四叶绿体的分离与观察
实验四 叶绿体的分离与观察
实验四叶绿体的分离与观察
实验四 叶绿体的分离与观察
9.4IsolationandCharacterizationofCellOrganelles（细胞器）Centrifugationcan separatemanytypes of oganellesfromcellhomogenatearmoredchambersedimentingmaterialrotorrefrigerationvacuummotor
9.4 Isolation and Characterization of Cell Organelles (细胞器) Centrifugation can separate many types of oganelles from cell homogenate
9.4IsolationandCharacterizationofCell Organelles（细胞器）Centrifugation can separatemanytypes ofoganellesfromcellhomogenate→Fractionation（细胞组分的分级分离）Centrifugation techniques:(1）Differentialcentrifugation（差速离心）(2）Density-gradientcentrifugation（密度梯度离心
9.4 Isolation and Characterization of Cell Organelles (细胞器) Centrifugation can separate many types of oganelles from cell homogenate Fractionation (细胞组分的分级分离). Centrifugation techniques: (1) Differential centrifugation (差速离心) (2) Density-gradient centrifugation (密度梯度离心)
9.4IsolationandCharacterizationofCellOrganelles（细胞器）Centrifugationcanseparatemanytypes ofoganellesfrom cellhomogenate,1st Step:Differential centrifugationFilterhomogenateto removeclumpsofunbrokencells,connectivetissue,etc.CentrifugeSupernatant(上清液)Pourout:rout:Pourout:100,0300,000gx15bo0gxDogx10min2hPourout5hin60mmPlasmaLRibosomalPelletSolubleFiltorNucleiLMitochondria,partofmembrane,chloroplasts,subunits,homogenate(颗粒团)microsomalsmallcytoplasmlysosomes,fractionandpolyribo-(cytosol)(fragmentsofsomesperoxisomesendoplasmicreticulum)and largepolyribosomes沉降顺序：细胞核一线粒体,溶酶体与过氧化物酶体一内质网与高尔基体一核糖体
9.4 Isolation and Characterization of Cell Organelles (细胞器) Centrifugation can separate many types of oganelles from cell homogenate, 1st Step: Differential centrifugation 沉降顺序：细胞核—线粒体,溶酶体与过氧化物酶体—内质网与高尔基体—核糖体。 Pellet (颗粒团) Supernatant (上清液)