RNA polymerase dunng normal el ongation 9.2 Transcription is catalyzed by RNA polymerase RNA polymerase is stalled backtracks region of RNA is cleaved Cleavage Figure 9.6 A stalled New 3 end is located in catalytic site RNa polymerase can be released by cleaving the 3 end Catalytic site resum es elongation of RNA of the transcript 消当
Figure 9.6 A stalled RNA polymerase can be released by cleaving the 3￠ end of the transcript. 9.2 Transcription is catalyzed by RNA polymerase
9. 3 RNA polymerase consists of multiple subunits Promoter is a region of dNa involved in binding of rna polymerase to initiate transcription Terminator is a sequence of DNa, represented at the end of the transcript, that causes rna polymerase to terminate transcription 消当
Promoter is a region of DNA involved in binding of RNA polymerase to initiate transcription. Terminator is a sequence of DNA, represented at the end of the transcript, that causes RNA polymerase to terminate transcription. 9.3 RNA polymerase consists of multiple subunits
Temp late recognition RNA polymerase binds to duplex DNA 9.3 RNA polymerase consists of multiple DNA is unwound at pro moter subunits Initia tion: Chains of 2-9 bases are synthesized and released Figure 9. 7 Transcription has Elongati on. RNA polymerase synthesizes RNA four stages, which involve different types of interaction between RNa polymerase and Unwound region moves w ith RNA polym erase DNA. The enzyme binds to the 入AO promoter and melts DNA, RNA polymerase reaches end of gene remains stationary during initiation, moves along the template durign elongation, and Termination RNA polymerase and RNA are rel eased dissociates at termination 消当
Figure 9.7 Transcription has four stages, which involve different types of interaction between RNA polymerase and DNA. The enzyme binds to the promoter and melts DNA, remains stationary during initiation, moves along the template durign elongation, and dissociates at termination. 9.3 RNA polymerase consists of multiple subunits
Gene product Functions 9.3 RNA polymerase enzvme assem bl ooA 2 a subunits promoter recognition consists of multiple (40kD each binds some activ ators subunits poB B subunit 155kD) cataltic center rpoc subunit Figure 9. 8 Eubacterial 160KD RNa polymerases have s subunit four types of subunit; a, B 7(32-90KD) promoter specifi city and b have rather constant sizes in different bacterial species, but o varies more widely E coi enzyme =465 KD 消当
Figure 9.8 Eubacterial RNA polymerases have four types of subunit; a, b, and b have rather constant sizes in different bacterial species, but s varies more widely. 9.3 RNA polymerase consists of multiple subunits
9. 4 Sigma factor controls binding to DNA Sigma factor is the subunit of bacterial RNA polymerase needed for initiation; is the major influence on selection of binding sites(promoters) 消当
Sigma factor is the subunit of bacterial RNA polymerase needed for initiation; is the major influence on selection of binding sites (promoters). 9.4 Sigma factor controls binding to DNA