Chapter 22 Nuclear splicing 莘大
Chapter 22 Nuclear splicing
22.1 Introduction 22.2 Nuclear splice junctions are short sequences 22.3 Splice junctions are read in pairs 22. 4 Nuclear splicing proceeds through a lariat 22.5 snRNAS are required for splicing 22.6 UI snRNP initiates splicing 22.7 The E complex can be formed in alternative ways 22. 8 5 snRNPs form the spliceosome 22.9 An alternative splicing apparatus uses different snRNP 22 10 Group II introns autosplice via lariat formation 22. 11 Alternative splicing involves differential use of splice junctions 22 12 CiS-splicing and trans-splicing reactions 22 13 Yeast tRNA splicing involves cutting and rejoining 22 14 The unfolded protein response is related to tRNA splicing 22. 15 The 3c ends of poll and pollll transcripts are generated by termination 22.16 The 3c ends of mRNAS are generated by cleavage 22 17 Cleavage of the 3c end may require a small rNA 22.18 Production of rRNA requires cleavage and modification events 22 19 Small RNAs are required for rRNA processing 消当
22.1 Introduction 22.2 Nuclear splice junctions are short sequences 22.3 Splice junctions are read in pairs 22.4 Nuclear splicing proceeds through a lariat 22.5 snRNAs are required for splicing 22.6 U1 snRNP initiates splicing 22.7 The E complex can be formed in alternative ways 22.8 5 snRNPs form the spliceosome 22.9 An alternative splicing apparatus uses different snRNPs 22.10 Group II introns autosplice via lariat formation 22.11 Alternative splicing involves differential use of splice junctions 22.12 cis-splicing and trans-splicing reactions 22.13 Yeast tRNA splicing involves cutting and rejoining 22.14 The unfolded protein response is related to tRNA splicing 22.15 The 3¢ ends of polI and polIII transcripts are generated by termination 22.16 The 3¢ ends of mRNAs are generated by cleavage 22.17 Cleavage of the 3¢ end may require a small RNA 22.18 Production of rRNA requires cleavage and modification events 22.19 Small RNAs are required for rRNA processing
21.1Introduction RNA Splicing is the process of excising the sequences in RNa that correspond to introns, so that the sequences corresponding to exons are connected into a continuous mrNA pre-mRNA heterogeneous nuclear RNA (hnRNA) hnRNP 消当
RNA splicing is the process of excising the sequences in RNA that correspond to introns, so that the sequences corresponding to exons are connected into a continuous mRNA. pre-mRNA heterogeneous nuclear RNA (hnRNA). hnRNP 21.1 Introduction
22. Introduction Figure 22.1 hnRNP hnrna 200A 40S exists as a ribonuclear otein particle 500-800 bases RNA organized as Core proteins (-30-40 KD) a series of b A1A2日1日2C1c2 eads 请莘大
Figure 22.1 hnRNA exists as a ribonucleopr otein particle organized as a series of beads. 22.1 Introduction
xon bren I Exam2余ton2om3nnEo0年mto(n5 22.1 Introduction WNF A i Figure 22.2 RNA is modified in the nucleus by Endd'modrfication additions to the5￠and3￠ ends and by splicing to remove the introns. The NUCLEUS splicing event requires breakage of the exon-intron ransport junctions and joining of the ends of the exons the Transation: CYTOPLASM expanded illustration shows the principle schematically but not the actual order of exon events. Mature mRNA is Exon-intron junctions are b ken transported through nuclear pores to the cytoplasm. Exons are joined where it is translated 请莘大
Figure 22.2 RNA is modified in the nucleus by additions to the 5￠ and 3￠ ends and by splicing to remove the introns. The splicing event requires breakage of the exon-intron junctions and joining of the ends of the exons; the expanded illustration shows the principle schematically, but not the actual order of events. Mature mRNA is transported through nuclear pores to the cytoplasm, where it is translated. 22.1 Introduction