9.2 Transcription 入A is catalyzed by RNA polymerase Figure 9.3 Transcription takes place in a bubble, in which RNa is synthesized 入OO by base pairing with one strand ofdna in the transiently unwound region As the bubble progresses NA the dna duplex reforms behind it. displacing the RNA in the form of a single polynucleotide chain 八AO 消当
Figure 9.3 Transcription takes place in a bubble, in which RNA is synthesized by base pairing with one strand of DNA in the transiently unwound region. As the bubble progresses, the DNA duplex reforms behind it, displacing the RNA in the form of a single polynucleotide chain. 9.2 Transcription is catalyzed by RNA polymerase
9.2 Transcription Enzym e movement is catalyzed by RNA polymerase DNA coding strand Rewind ing point Unwinding point Figure 9.4 During transcription, the bubble is maintained within bacterial RNA polymerase which unwinds and rewinds dna. maintains NNA template strand the conditions of the partner and template DNA strands, and synthesizes Catalvtic site RNA RNA hind ing site, 消当
Figure 9.4 During transcription, the bubble is maintained within bacterial RNA polymerase, which unwinds and rewinds DNA, maintains the conditions of the partner and template DNA strands, and synthesizes RNA. 9.2 Transcription is catalyzed by RNA polymerase
9.2 Transcription is catalyzed by RNA polymerase Figure 9.5 Yeast RNA polymerase has grooves that could be binding sites for nucleic acids The pink beads show a possible path for DNA that is 25 A wide and 5-10 A deep. The green beads show a narrower channe 消当
Figure 9.5 Yeast RNA polymerase has grooves that could be binding sites for nucleic acids. The pink beads show a possible path for DNA that is ~25 Å wide and 5-10 Å deep. The green beads show a narrower channel. 9.2 Transcription is catalyzed by RNA polymerase
5 end of polynucleotide chain phosphate 9.2 Transcription being added to chain is catalyzed by RNA polymerase O=P Figure 9.6 Phosphodiester bond formation involves a hydrophilic attack by the 0=P.O 3-OH group of the last nucleotide of the chain on OsP.O O=P·O the 5 triphosphate of the Pyrophosphate incoming nucleotide. with release of pyrophosphate 消当
Figure 9.6 Phosphodiester bond formation involves a hydrophilic attack by the 3’-OH group of the last nucleotide of the chain on the 5’ triphosphate of the incoming nucleotide, with release of pyrophosphate. 9.2 Transcription is catalyzed by RNA polymerase
9.2 Transcription is catalyzed by RNA polymerase ac en of enyme moves one 雷备备备备 Figure 9. RNA polymerase moves Back end continues Frorm and like an inchworm se added to RNA 器器 during elongation when it compresses from the back end and release from the front end 消当
Figure 9.6 RNA polymerase moves like an inchworm during elongation, when it compresses from the back end and release from the front end. 9.2 Transcription is catalyzed by RNA polymerase