Rifampicin inhibits DNA-dependent RNA polymerase in bacterial cells Mechanism: by binding its B-subunit of RNA polymerase Bactericidal antibiotic Separate Rifampicin sensitive 1 Rebuild Rifampicin resistance Rifampicin resistance
Rifampicin inhibits DNA-dependent RNA polymerase in bacterial cells Mechanism: by binding its β-subunit of RNA polymerase Bactericidal antibiotic Rifampicin sensitive Rifampicin resistance Rifampicin resistance Separate Rebuild
S 1.1 RNA polymerase-RNA pol b.RNA pol in eukaryote types function Sensivity to a-amanitine RNA pol I 45S rRNA insensitive RNA pol II mRNA many snRNA sensitive tRNA RNA pol III 5S rRNA moderately sensitive some snRNA Mt RNA pol RNA in Mt “death cap”mushroom TFIF RNA polymerase ll CTD:carboxyl terminal domain-,c-terminus of the largest subunit of RNA polII,which consists of repeat sequence-Tyr Ser Pro Thr Ser Pro Ser. Tyr ,Ser and Thr residues in CTD can be phorsphorylated,which is involved in initiation of transcription. CTD RNA polII
§1.1 RNA polymerase- RNA pol b. RNA pol in eukaryote types function Sensivity to α-amanitine RNA pol I 45S rRNA insensitive RNA pol II mRNA many snRNA sensitive RNA pol III tRNA 5S rRNA some snRNA moderately sensitive Mt RNA pol RNA in Mt CTD: carboxyl terminal domain -, c-terminus of the largest subunit of RNA polⅡ , which consists of repeat sequence-Tyr Ser Pro Thr Ser Pro Ser. Tyr ,Ser and Thr residues in CTD can be phorsphorylated, which is involved in initiation of transcription. “death cap” mushroom RNA polⅡ
Characteristics of RNA pol o No primer No exonuclease activity-no proofreading function
Characteristics of RNA pol ◦ No primer ◦ No exonuclease activity-no proofreading function
S 1.2 DNA template Structural gene codes for any RNA or protein product. template strand:antisense strand.Template for guiding RNA synthesis. coding strand:sense strand.Strand complementary to template strand,same with RNA product. Structural gene Transcription direction 5 coding strand template strand| 3 template strand coding strand Transcription direction
§1.2 DNA template 5 3 coding strand template strand template strand coding strand Structural gene Transcription direction Transcription direction • Structural gene : codes for any RNA or protein product. • template strand: antisense strand. Template for guiding RNA synthesis. • coding strand: sense strand. Strand complementary to template strand, same with RNA product
5'.…GCAGTACATGTC3' Coding strand 3'…cgtgatg tacag…5'Template strand Transcription 5'.…GCAGUACAUGUC3' mRNA Translation N…Ala·Val·His·Val…C Protein mRNA:sequence is the same to coding strand
5′···GCAGTACATGTC ···3′ 3′··· c g t g a t g t a c a g ···5′ 5′···GCAGUACAUGUC ···3′ N······Ala ·Val · His · Val ······C Coding strand Template strand mRNA Protein Transcription Translation mRNA: sequence is the same to coding strand