28S rRNA 18S rRNA 5 S,5.8S,tRNA Electrophoresis of total RNA from eukaryotes
Electrophoresis of total RNA from eukaryotes
Section1 Transcription system
Section1 Transcription system
Transcription system Substrates:NTP(ATP,UTP,GTP,CTP) Template:DNA Enzyme:RNA polymerase,RNA-pol Other protein factors
Transcription system Substrates: NTP (ATP, UTP, GTP, CTP) Template: DNA Enzyme: RNA polymerase, RNA-pol Other protein factors
S1.1 RNA polymerase-RNA pol a.RNA pol in prokaryote In prokaryotic (bacteria)all types of RNA are synthesized by only one species of RNA polymerase
§1.1 RNA polymerase- RNA pol In prokaryotic (bacteria) all types of RNA are synthesized by only one species of RNA polymerase. a. RNA pol in prokaryote
Subunits of RNA polymerase in prokaryote subunit gene MW number function rp0A40000 2 Binding upper promoter element and regulatory factors B rp0B155000 1 Polymerase activity B rp0C160000 Binding to DNA(nonspecifically) 0 rp0D32000-920001 Binding specific promoter regions 0 9000 Not known Synthesize RNA B Promoter recognization 670:common promoter 032 HSP promoter Transcription specificity Core enzyme Holoenzyme
subunit gene MW number function α rpo A 40000 2 Binding upper promoter element and regulatory factors β rpo B 155000 1 Polymerase activity β′ rpo C 160000 1 Binding to DNA(nonspecifically) σ rpo D 32000-92000 1 Binding specific promoter regions ω 9000 Not known Subunits of RNA polymerase in prokaryote Core enzyme Holoenzyme Synthesize RNA Transcription specificity Promoter recognization σ 70 : common promoter σ 32 : HSP promoter