Systems for the Propagation of Viruses Peopl de Animals: cOWS, chickens, mice, rats, suckling mice Embryonated eggs Organ and tissue culture Organ culture Primary tissue culture Cell lines: diploid Tumor or immortalized) cell line
11 Systems for the Propagation of Viruses • People • Animals: cows, chickens, mice,rats, suckling mice • Embryonated eggs • Organ and tissue culture Organ culture Primary tissue culture Cell lines: diploid Tumor or （immortalized ）cell line
Viral detection CPE ° Hemadsorption Interfere · Metabo| ize of cell
12 Viral detection • CPE • Hemadsorption • Interfere • Metabolize of cell
TCID50 (Tissue culture infective dose) TCID50 is defined as that dilution of virus which will cause CPE in 50% of a given batch of cell culture TCID50= logo of highest dilution giving 100%CPE +1/2 (total number of test units showing CPE)/ (number of test units per dilution)
13 TCID50 (Tissue culture infective dose) • TCID50 is defined as that dilution of virus which will cause CPE in 50% of a given batch of cell culture • TCID50= log10 of highest dilution giving 100%CPE +1/2 – (total number of test units showing CPE)/ (number of test units per dilution)
Viral identification Complement fixation: Hemagglutination inhibition Neutralization Immunofluorescence( direct or indirect) Latex agglutination In situ elA ELISA RIA(radioimmuno
14 Viral identification • Complement fixation： • Hemagglutination inhibition • Neutralization • Immunofluorescence ( direct or indirect) • Latex agglutination • In situ EIA • ELISA • RIA(radioimmuno
Laboratory Diagnosis Microscopy Identification Virus isolation and identification Detection of viral proteins( antigens and enzymes) Detection of viral genetic material Serologic procedures
15 Laboratory Diagnosis • Microscopy Identification • Virus isolation and identification • Detection of viral proteins( antigens and enzymes) • Detection of viral genetic material • Serologic procedures