Chlamydiae 2021/2/21 microbiology 8-year course
2021/2/21 microbiology 8-year course 1 Chlamydiae
O Biological Features Structure and chemical composition ◆ Developmental cycle ◆ Staining properties ◆ Antigens ◆ Growth and metabolism Characteristics of host-parasite relationship ◆ Classification 2021/2/21 microbiology 8-year course
2021/2/21 microbiology 8-year course 2 Biological Features Structure and chemical composition Developmental cycle Staining properties Antigens Growth and metabolism Characteristics of host-parasite relationship Classification
O Structure and chemical composition a nonreplicating, fectious particle called the elementary E.B. body(eB) . an intracytoplasmic form called the reticulate body(RB 2021/2/21 microbiology 8-year course
2021/2/21 microbiology 8-year course 3 Structure and chemical composition a nonreplicating, infectious particle called the elementary body (EB) an intracytoplasmic form called the reticulate body (RB)
O Structure and chemical composition (Foto: Prof. Eiko Petersen, Universitat Freiburg/Brsg) To be seen each cell are two nclusions with elementary bodies (Giemsa stain) 2021/2/21 microbiology 8-year course
2021/2/21 microbiology 8-year course 4 Structure and chemical composition
O Structure and chemical composition The elementary body, which is covered by a rigid cell wall, contains a DNA genome with a molecular weight of 66 X 107(about 600 genes, one-quarter of the genetic information present in the DNA of Escherichia coli) A cryptic DNA plasmid(7, 498 base pairs) is also found. It contains an open reading frame for a gene involved in DNA replication the elementary body contains an RNA polymerase responsible for the transcription of the DNA genome after entry into the host cell cytoplasm and the initiation of the growth cycle Ribosomes and ribosomal subunits are present in the elementary bodies Throughout the developmental cycle, the DNA genome, proteins, and ribosomes are retained in the membrane-bound reticulate body 2021/2/21 microbiology 8-year course
2021/2/21 microbiology 8-year course 5 Structure and chemical composition The elementary body, which is covered by a rigid cell wall, contains a DNA genome with a molecular weight of 66 X 107 (about 600 genes, one-quarter of the genetic information present in the DNA of Escherichia coli). A cryptic DNA plasmid (7,498 base pairs) is also found. It contains an open reading frame for a gene involved in DNA replication. the elementary body contains an RNA polymerase responsible for the transcription of the DNA genome after entry into the host cell cytoplasm and the initiation of the growth cycle. Ribosomes and ribosomal subunits are present in the elementary bodies. Throughout the developmental cycle, the DNA genome, proteins, and ribosomes are retained in the membrane-bound reticulate body