Mycobacterium
Mycobacterium
MYCOBACTERIUM THIS GENUS S COMPOSED OF Strictly aerobic, acid-fast rods, does not Stain well(gram stain indeterminant), DNA has high g+c content, unique cell wall, Mycolic acid carbon chain length>c60 Relatively slow growth(two groups) A. RAPID GROWERS (Visible colonies in <5 days B SLOW GROWERS (Visible colonies in 5 days) TYPE SPECIES: Mycobacterium tuberculosis
MYCOBACTERIUM THIS GENUS IS COMPOSED OF: Strictly aerobic, acid-fast rods, does not Stain well (gram stain indeterminant), DNA has high g+c content, unique cell wall, Mycolic acid carbon chain length > c60 Relatively slow growth (two groups) A. RAPID GROWERS (Visible colonies in <5 days) B. SLOW GROWERS (Visible colonies in > 5 days) TYPE SPECIES: Mycobacterium tuberculosis
THE GENUS MYCOBACTERIUM CAN BE DIVIDED INTO FOUR BROAD GROUPS 1. THE TUBERCULOSIS COMPLEX 2. SLOW GROWING MYCOBACTERIA OTHER THAN TUBERCULOSIS (MOTT) 3. RAPIDLY GROWING MYCOBACTERIA 4 MYCOBACTERIUM LEPRAE
THE GENUS MYCOBACTERIUM CAN BE DIVIDED INTO FOUR BROAD GROUPS 1. THE TUBERCULOSIS COMPLEX 2. SLOW GROWING MYCOBACTERIA OTHER THAN TUBERCULOSIS (MOTT) 3. RAPIDLY GROWING MYCOBACTERIA 4. MYCOBACTERIUM LEPRAE
Acid Fastness Stain (∠ieh- Neelsen stain) o flood the slide with basic fuchsin(a red dye )in 5% phenol as a mordant o heat gently for few minutes to melt the wax e wash with 3% Hcl in ethanol o counter-stain with methylene blue Mycobacterium stains red and other bacteria and the background are blue The mycolic acid and its derivatives are responsible for the acid
Acid Fastness Stain (Ziehl-Neelsen stain) ⚫ flood the slide with basic fuchsin (a red dye) in 5% phenol as a mordant. ⚫ heat gently for few minutes to melt the wax. ⚫ wash with 3% HCl in ethanol. ⚫ counter-stain with methylene blue. Mycobacterium stains red and other bacteria and the background are blue. The mycolic acid and its derivatives are responsible for the acid f
THE TUBERCULOSIS COMPLEX Organisms that resemble M. tuberculosis, Causing a similar type of disease in humans) 1 tuberculosis 2 M bovis
THE TUBERCULOSIS COMPLEX (Organisms that resemble M. tuberculosis; Causing a similar type of disease in humans) 1. M. tuberculosis 2. M. bovis