Cell death Cell rounding Degeneration Aggregation Loss of attachments to substrate Characteristic histological changes inclusion bodies in the nucleus or cytoplasm, margination of chromatin Syncytia: multinucleated giant cells caused by virus induced cell-cell fusion
6 • Cell death Cell rounding Degeneration Aggregation Loss of attachments to substrate • Characteristic histological changes:inclusion bodies in the nucleus or cytoplasm, margination of chromatin • Syncytia: multinucleated giant cells caused by virusinduced cell-cell fusion
Fluorescent microscopy Fluorescent-antibody staining
7 Fluorescent microscopy • Fluorescent-antibody staining
Electron microscopy Direct detection: Human rotavirus: HAV HBV, Smallpox virus; Herpes virus Immune Electron micros copy Human rotavirus: HAV
8 Electron microscopy • Direct detection : Human rotavirus; HAV; HBV; Smallpox virus; Herpes virus. • Immune Electron microscopy: Human rotavirus; HAV;
Laboratory Diagnosis Microscopy Identification Virus isolation and identification Detection of viral proteins( antigens and enzymes )] Detection of viral genetic material Serologic procedures
9 Laboratory Diagnosis • Microscopy Identification • Virus isolation and identification • Detection of viral proteins( antigens and enzymes)] • Detection of viral genetic material • Serologic procedures
Viral isolation and dentification Viral growth and Cell culture Viral detection Viral Identification Interpretation of culture results
10 Viral isolation and Identification • Viral Growth and Cell culture • Viral Detection • Viral Identification • Interpretation of culture results