20.2 Eukaryotic RNA polymerases consist of many subunits Size No. Features kD 1 Related to bacterial subunit B' 200 binds DNA has CTD =(YSPTSPS) [yeast n=26;mouse n =52] 1 Related to bacterial subunit B 100 binds nucleotides 50 Figure 20.2 2 Related to bacterial subunito Eukaryotic RNA < 2 polymerase II Common to all 3 polymerases 25 1 Common to all 3 polymerases has >10 subunits. 2 Common to all 3 polymerases 清苇大当
Figure 20.2 Eukaryotic RNA polymerase II has >10 subunits. 20.2 Eukaryotic RNA polymerases consist of many subunits
20.3 Promoter elements are defined by mutations and footprinting Cotransfection is the simultaneous transfection of two markers. 清菜大当
Cotransfection is the simultaneous transfection of two markers. 20.3 Promoter elements are defined by mutations and footprinting
Upstream Promoter Transcribed region 20.3 Promoter 八入入八八NN elements are defined N八八八八N by mutations and Delete and reconnect footprinting NN八N入NNN Figure 20.3 Promoter boundaries Transcription can be determined by making RNA still made: deletions that progressively deletion does not enter promoter remove more material from one N入八八入入八八 side.When one deletion fails to Delete and reconnect prevent RNA synthesis but the next stops transcription,the NiN八入NN boundary of the promoter must lie between them. No RNA made:deletion has entered promoter 清第大当 Upstream boundary of promoter lies between ends of deletions
Figure 20.3 Promoter boundaries can be determined by making deletions that progressively remove more material from one side. When one deletion fails to prevent RNA synthesis but the next stops transcription, the boundary of the promoter must lie between them. 20.3 Promoter elements are defined by mutations and footprinting
P ol I prom oters have two sequence components 20.4 RNA CTCCGAGTCGNNNNNNTGGGC Startpoint polymerase I has a Upstream control element Core prom oter -170160-150-140.130120.110 -605040-30-2010+10+20 bipartite promoter 入NM八NNN八N八八八八八 The initiation complex assembles in three stages Figure 20.4 Transcription units UBFI UBFI binds to G-C-rich sequence for RNA polymerase I have a core promoter separated by ~70 bp from the upstream control 八八代NN八八八入心 element.UBF1 binds to both SL1 SL1 binds cooperatively with UBF1 regions,after which SLI can bind.RNA polymerase I then binds to the core promoter.The N入NN入八入 nature of the interaction between the factors bound at the Poll upstream control element and those at the core promoter is not N八NNN入NNN known 情華大当
Figure 20.4 Transcription units for RNA polymerase I have a core promoter separated by ~70 bp from the upstream control element. UBF1 binds to both regions, after which SL1 can bind. RNA polymerase I then binds to the core promoter. The nature of the interaction between the factors bound at the upstream control element and those at the core promoter is not known. 20.4 RNA polymerase I has a bipartite promoter
20.5 RNA polymerase III uses both downstream and upstream promoters Preinitiation complex in eukaryotic transcription describes the assembly of transcription factors at the promoter before RNA polymerase binds. 清菜大当
Preinitiation complex in eukaryotic transcription describes the assembly of transcription factors at the promoter before RNA polymerase binds. 20.5 RNA polymerase III uses both downstream and upstream promoters