Diagrammatic representation of the structure of virus particles e M-protein 王3 RNA O 180nm transcript ingle tranded RNA and proteins 9的的 9999999 Rabies virus(and H(R have tight-fitting envelopes
180nm Diagrammatic representation of the structure of virus particles RNA Reverse transcriptase Lipid membrane 100nm Rabies virus(l)and HIV(R) have tight-fitting envelopes
grammatic representation of the structure of virus particles Glycoprotein envelope LO 90nm Icosahedral capsId containing DNA herpes simplex()has a loose-fitting envelope, whereas adenovirus(R)is non-enveloped
herpes simplex (l) has a loose-fitting envelope, whereas adenovirus (R) is non-enveloped. Diagrammatic representation of the structure of virus particles Glycoprotein envelope Icosahedral capsid containing DNA 90nm 45nm
2. Methods of study (1) While the electron microscope(EM) had been known for many years, the invention of the negative-staining technique in 1959 revolutionized studies on virus structure In negative-contrast EM virus particles are mixed with a heavy metal solution(e.g. sodium phosphotungstate磷钨酸盐) and dried onto a support film.The stain provides an electron-opaque background against which the virus can be visualized Negative-staining technique
While the electron microscope (EM) had been known for many years, the invention of the negative-staining technique in 1959 revolutionized studies on virus structure. In negative-contrast EM, virus particles are mixed with a heavy metal solution (e.g. sodium phosphotungstate磷钨酸盐) and dried onto a support film. The stain provides an electron-opaque background against which the virus can be visualized。 2. Methods of study（1） Negative-staining technique
Methodes of study (2) Observation under the electron microscope has thus allowed the definition of virus morphology at the 50 77A resolution level. In addition, negative staining of thin sections of infected cells has allowed definition of structures which appear during virus maturation and their interactions with cellular proteins Electron microscopy
Observation under the electron microscope has thus allowed the definition of virus morphology at the 50- 77A resolution level. In addition, negative staining of thin sections of infected cells has allowed definition of structures which appear during virus maturation and their interactions with cellular proteins. Methodes of study （2） Electron microscopy
Methods of study(3) Immunoelectron microscopy is used to study those viruses which may be present in low concentrations or grow poorly in tissue culture (e. g. Norwalk virus). These clumps of virus are more readily observed Immunoelectron microscopy
Immunoelectron microscopy is used to study those viruses which may be present in low concentrations or grow poorly in tissue culture (e.g. Norwalk virus). These clumps of virus are more readily observed. Methods of study（3） Immunoelectron microscopy