TISSUE ENGINEERING What is tissue engineering Production of tissue in vitro by growing cells in porous, absorbable scaffolds (matrices). Why is tissue engineering necessary? Most tissues cannot regenerate when injured or diseased

SACHUSE Massachusetts Institute of Technology Harvard Medical School Brigham and Women's/Massachusetts General Hosp

C. Synthesis of biologically active scaffolds (regeneration templates) 1. History of biologically active scaffolds (regeneration templates). 2. Physical chemistry of collagen: Melting of collagen quaternary structure (thromboresistance). Melting of collagen tertiary structure (gelatinization). 3. Synthesis of ECM analogs: lonic complexation

The Extracellular Matrices Part. 2. Elastin fibers. 3. Proteoglycans(PG) and glycosaminoglycans (GAG). 4. Cell-adhesion molecules (CAM)

RESPONSE TO IMPLANTS: WOUND HEALING Surgical Implantation Inc. time Vascular Response Acute Clotting Inflammation Phagocytosis Granulation Neovascularization New Collagen Synthesis Tissue

biologically active model of ECM acts as an insoluble regulator of cell function image removed due to copyright considerations

RESPONSE TO IMPLANTS WOUND HEALING flury Vascular Response Inflammation Tissue of labile Tissue of and stable cells Permanent cells Framework framework Scar Intact Destroyed

Outline of three lectures on Irreversible Healing of Extracellular Matrix. A. Irreversible healing of ECMs in different organs. B. Structure and function of naturally occurring ECMs. C. Synthesis of biologically active ECM models

2.4.1 Cell Activities \Packaged\ sequences (cascades) of cell activities/functions with an identifiable beginning and an end 2.4.1.1 Mitosis(Proliferation) Mitosis is a complex of processes leading to the division of a cell such that the two daughter nuclei receive identical complements of the number of chromosomes characteristic of the cells of the species. All cells arise from the division of pre-existing cells. The process of mitosis is divided into four phases:

UNIT CELL PROCESSES Regulator UCP Cell Matrix Product Regulator Connect. ECM Mitosis Tiss. Adhesion Sy Synthesis Epithelia |Protein Migration Muscle Collagen