Buchner.兄弟的试验： 用细砂研磨酵母细胞，压取汁液，汁液不 含活细胞，但仍能使糖发酵生成酒精和二氧 化碳。 证明：发酵与细胞的活动无关
Buchner兄弟的试验： 用细砂研磨酵母细胞，压取汁液，汁液不 含活细胞，但仍能使糖发酵生成酒精和二氧 化碳。 证明：发酵与细胞的活动无关
The Nobel Prize in Chemistry 1907 "for his biochemical researches and his discovery of cell-free fermentation" Eduard Buchner Germany Landwirtschaftliche Hochschule (Agricultural College)Berlin,Germany b.1860 d.1917
The Nobel Prize in Chemistry 1907 "for his biochemical researches and his discovery of cell-free fermentation" b. 1860 d. 1917 Landwirtschaftliche Hochschule (Agricultural College) Berlin, Germany Germany Eduard Buchner
酶一活细胞产生的，能在细胞内外起作用 的（催化）生理活性物质。 enzyme-organic chemical substance (a catalyst)formed in living cells,able to cause changes in other substance without being changed itself. 酶的化学本质？
酶——活细胞产生的，能在细胞内外起作用 的（催化）生理活性物质。 enzyme—organic chemical substance (a catalyst) formed in living cells, able to cause changes in other substance without being changed itself. 酶的化学本质？
3.酶学的迅速发展（理论研究) (1)酶的化学本质 ·德国化学家R.Willstatter的错误观点。 ·1926年，美国康乃尔大学的"独臂学者”Sumner (萨姆纳)博士从刀豆中提取出脲酶结晶，并证明具 有蛋白质的性质。 ·1930年，美国的生物化学家Northrop:分离得到了胃 蛋白酶(pepsin)、胰蛋白酶(trypsin)、胰凝乳蛋 白酶(chymotrypsin)结晶，确立了酶的化学本质
3.酶学的迅速发展（理论研究） （1） 酶的化学本质 •德国化学家R.Willstatter的错误观点。 •1926年,美国康乃尔大学的“独臂学者” Sumner （萨姆纳）博士从刀豆中提取出脲酶结晶，并证明具 有蛋白质的性质。 •1930年，美国的生物化学家Northrop分离得到了胃 蛋白酶（pepsin）、胰蛋白酶（trypsin）、胰凝乳蛋 白酶（chymotrypsin）结晶，确立了酶的化学本质
The Nobel Prize in Chemistry 1946 "for his discovery "for their preparation of enzymes and that enzymes virus proteins in a pure form" can be crystallized" James Batcheller John Howard Wendell Meredith Sumner Northrop Stanley 1/2 of the prize 1/4 of the prize 1/4 of the prize Cornell University Rockefeller Institute for Medical Rockefeller Institute for Medical Ithaca,NY,USA Research Research Princeton,NJ,USA Princeton,NJ,USA 1887-1955 1891-1987 1904-1971
The Nobel Prize in Chemistry 1946 "for their preparation of enzymes and virus proteins in a pure form" “ for his discovery that enzymes can be crystallized" 1887-1955 1891-1987 1904-1971 Rockefeller Institute for Medical Research Princeton, NJ, USA Rockefeller Institute for Medical Research Princeton, NJ, USA Cornell University Ithaca, NY, USA 1/2 of the prize 1/4 of the prize 1/4 of the prize Wendell Meredith Stanley John Howard Northrop James Batcheller Sumner