DNA的结构 DNA The structure of dna Molecule: WO Views Adenine and Phosphate Axis of helix Sugar-phosphate H"- 5
、理论基础 ●2.基因是可切割的; ●限制性内切酶( Restriction Enzyme),如 ECORI, hindl等
l 2. 基因是可切割的； l 限制性内切酶（Restriction Enzyme），如EcoRI，HindIII等
EcoRI DNHEAE THHDOXX DNA fragments ioin et sticky ends sticky end ZXHEA BooO sticky end Restriction Enzyme Action of ecori
Enzyme Sequence of Microbial origin 限制性内物酸 Recognition Site Thermus aquaticus YTI Rsal Rhodopseudomonas sphaeroides 5 W. Arberfa H O. Smith sau3aI 5GATc 3 Staphylococcus aureUs 3A CITA 5eAT国3 Escherichia co c干A GGAITICIC 3CICITAIG G 5 amyloliquefaciens H 常用限制性内切酶 5AAGCTII haemophilus influenzae 种类及特性 3 TITICIGAA Kpnl Klebsiella pneumoniae CA TIGG 5 OK8 3 Caryophanon latum aCTIA.5 BssHIl stearothermophilus Noti 5'gaggclcl Nocardia otitidiscaviaru accaC s
常用限制性内切酶 种类及特性 W. Arber和H. O. Smith
其它工具酶 0连接酶 T4 DNA Ligase 1967 修补工具酶 DNA聚合酶I大片断 Klenow1971 末端加工酶 S1梭酸酶,碱性磷酸单脂酶 ●末端转移酶 人工加polA或poyT尾 0反转录酶
l连接酶 T4 DNA Ligase 1967 l修补工具酶 DNA聚合酶I 大片断 Klenow 1971 l末端加工酶 S1核酸酶 ， 碱性磷酸单脂酶 l 末端转移酶 人工加polyA 或polyT 尾 l 反转录酶