N-acetyl-glucosamine ●Mannose●Glucose 25.2 Oligosaccharides are added to proteins in Dolich the ER and Golgi ENDOPLASMIC RETICULUM Figure 25.3 An oligosaccharide e is formed on dolichol and Asparagine in target transferred by glycosyl transferase to asparagine of a target protein. 清菜大当 Glycoprotein
Figure 25.3 An oligosaccharide is formed on dolichol and transferred by glycosyl transferase to asparagine of a target protein. 25.2 Oligosaccharides are added to proteins in the ER and Golgi
N-acetyl-glucosamine Mannose●Glucose 25.2 Oligosaccharides are added to proteins in the HC-CHCNO ER and Golgi C=0 Figure 25.4 Sugars are ER glucosidases|&∥ removed in the ER in a fixed order,initially comprising 3 glucose and 1-4 mannose residues.This trimming ER mannosidase generates a high mannose oligosaccharide. High mannose oligosacchande 清菜大当
Figure 25.4 Sugars are removed in the ER in a fixed order, initially comprising 3 glucose and 1-4 mannose residues. This trimming generates a high mannose oligosaccharide. 25.2 Oligosaccharides are added to proteins in the ER and Golgi
Golgi mannosidase 25.2 Oligosaccharides are NH H HC-CH - added to proteins in the ER and Golgi Golgi N-acetyl- glucosamine transferase Figure 25.5 Processing for a complex oligosaccharide occurs in the Golgi and trims the original preformed unit to the Golgi mannosidase ll inner core consisting of 2 N-acetyl- glucosamine and 3 mannose residues. HC-CHa EndoH resistance Then further sugars can be added,in the C=0 order in which the transfer enzymes are Golgi enzymes add further residues encountered,to generate a terminal GalSialic acid region containing N-acetyl-glucosamine, HOCH-CN galactose,and sialic acid -08 nner cote Terminal region 清菜大兰
Figure 25.5 Processing for a complex oligosaccharide occurs in the Golgi and trims the original preformed unit to the inner core consisting of 2 N-acetylglucosamine and 3 mannose residues. Then further sugars can be added, in the order in which the transfer enzymes are encountered, to generate a terminal region containing N-acetyl-glucosamine, galactose, and sialic acid. 25.2 Oligosaccharides are added to proteins in the ER and Golgi
25.2 Oligosaccharides are added to proteins in the ER and Golgi transo Figure 25.6 The Golgi apparatus consists of a series of individual ￥0sG0lg membrane stacks. Photograph kindly R provided by Alain Rambourg. 清菜大当
Figure 25.6 The Golgi apparatus consists of a series of individual membrane stacks. Photograph kindly provided by Alain Rambourg. 25.2 Oligosaccharides are added to proteins in the ER and Golgi
25.2 Oligosaccharides are added to proteins in the ER and Golgi mannose-6-P receptors sialyl transterase N:(trans-Golgi netwo Figure 25.7 A Golgi acid phosphatase stack consists of a series galactosyl transferase of cisternae,organized with cis to trans polarity. 1P-NAc-glucosaminidase NADPase medial cistemna Protein modifications m annosidase ll occur in order as a glcN-acetyl-transferase mannosidase I protein moves from the 660cae cis face to the trans face. 清菜大兰
Figure 25.7 A Golgi stack consists of a series of cisternae, organized with cis to trans polarity. Protein modifications occur in order as a protein moves from the cis face to the trans face. 25.2 Oligosaccharides are added to proteins in the ER and Golgi