Developing of Chromatographic Technique 1.0 Cysteic Methionine sulphone Threo Serine acid Glutamic acid Glycine Alanine 0.5 Aspartic acid Cystine 0.1 Methionine sulphoxides 40 60 80 100 120140160180 200 220240 260280300320 150 cm column.pH 3.25.0.2 mol dm-a sodium citrate Eluate/ml 1.0 Lysine Histidine Leucine 05 Phenyl- 0.4 Tyrosine alanine Arginine 0.3 0.2 0.1 330350370390410.430450 470 .490 50 70 90 110 130 -pH 4.25.0.2 mol dm-3 sodium citrate- -15 cm column,pH 5.28,0.35- Eluate/ml mol dm-3 sodium citrate 1958,22 hours separation for 19 of amino acids
Developing of Chromatographic Technique 1958, 22 hours separation for 19 of amino acids
Developing of Chromatographic Technique 16 17 11 1415 12 8 9 10 7 6 18 5 9 18 Time/min 1982,less than 30 minutes separation for 18 of amino acids
Developing of Chromatographic Technique 1982, less than 30 minutes separation for 18 of amino acids
Schematic Diagram of Chromatography 气相色谱流程图 Flow or pressure controller Column Detector 山 Injection port or valve W Data station (Recorder) Carrier gas
Schematic Diagram of Chromatography 气相色谱流程图 Detector Carrier gas Flow or pressure controller Injection port or valve Column Data station W (Recorder)
Schematic Diagram of Chromatography 液相色谱流程图 Column PUMP Detector W Data station (Recorder) or Collection Mobile phase
Schematic Diagram of Chromatography 液相色谱流程图 PUMP Detector W Data station (Recorder) or Collection Column Mobile phase
色谱法的基本原理 Common Concepts and Principles of Chromatography a)All chromatographic separations are based on a common concept: The distribution of components in a mixture between two immiscible phases.one a stationary phase and another a movig (mobile)phase. However,for each chromatographic mode the nature of the physicochemical processes can be different. 混合物中各组分在互不相溶的固定相和流动相之间的 分配：而每一种形式的色谐分离的物理化学过程可以 是不同的
a) All chromatographic separations are based on a common concept: The distribution of components in a mixture between two immiscible phases, one a stationary phase and another a moving (mobile) phase. However, for each chromatographic mode the nature of the physicochemical processes can be different. 混合物中各组分在互不相溶的固定相和流动相之间的 分配；而每一种形式的色谱分离的物理化学过程可以 是不同的。 色谱法的基本原理 Common Concepts and Principles of Chromatography