A Brief History of Chromatography ☆Partition Chromatography(分配色谱) Liquid-Liquid Partition Chromatography ☆Plate Theory(塔板理论) the theoretical plate concept A gas might be used instead of a liquid in chromatography
A Brief History of Chromatography ☆ Partition Chromatography（分配色谱） Liquid-Liquid Partition Chromatography ☆ Plate Theory （塔板理论） the theoretical plate concept ☆ A gas might be used instead of a liquid in chromatography
A Brief History of Chromatography 1944,A.J.P.Martin,R.Cinsden and A.H.Gordon developed paper chromatography 1950,A.J.P.Martin and G.A.Howard developed reversed-phase chromatography 1952,A.J.P.Martin and A.T.James Gas-liquid chromatography 1952,A.J.P.Martin and R.L.M.Synge were awarded the Nobel Prize in Chemistry
A Brief History of Chromatography 1944, A.J.P. Martin, R. Cinsden and A.H. Gordon developed paper chromatography 1950, A.J.P. Martin and G.A. Howard developed reversed-phase chromatography 1952, A.J.P. Martin and A.T. James Gas-liquid chromatography 1952, A.J.P. Martin and R.L.M. Synge were awarded the Nobel Prize in Chemistry
A Brief History of Chromatography 1956,Rate theory,the Dutch scientist,Van Deemter 1963,HPLC was born,Giddings High Performance Liquid Chromatography High Pressure Liquid Chromatography Today
A Brief History of Chromatography 1956, Rate theory, the Dutch scientist, Van Deemter 1963, HPLC was born, Giddings High Performance Liquid Chromatography High Pressure Liquid Chromatography - - - Today
Developing of Chromatographic Technique 2.5 Leucine (a) 20 ('AInba) Isoleucine 05 Valine Methionine Tyrosine L 161 187 215 250 313 350 390415 450 15 200 300 Eluate/ml 2.0 Leucine Isoleucine 1.5 Alanine 1.0 Phenylalanine Glutan acid Valine Proline Threonine Methionine Aspa rtic Serine Ammonia 0.5 Lysine Tyrosine Glycine Arginine Histidine Cystine 13.5 2428 5259 7582 100106117 136 149 163 179 16.526 Solvent change Eluate/ml 1948,8 days separation for 16 amino acids
Developing of Chromatographic Technique 1948, 8 days separation for 16 amino acids