snRNPs √Small nuclear RNAs(核内小RNA)coupled to proteins are abbreviated as snRNPs, small nuclear ribonuclear proteins The snRNAs can be resolved on a gel: -U1,U2,U4,U5,U6 -All 5 snRNAs join the spliceosome to play crucial roles in splicing 14-11
14-11 snRNPs ✓Small nuclear RNAs (核内小RNA) coupled to proteins are abbreviated as snRNPs, small nuclear ribonuclear proteins ✓The snRNAs can be resolved on a gel: – U1, U2, U4, U5, U6 – All 5 snRNAs join the spliceosome to play crucial roles in splicing
snRNPs √Small nuclear RNAs(核内小RNA)coupled to proteins are abbreviated as snRNPs, small nuclear ribonuclear proteins The snRNAs can be resolved on a gel: -U1,U2,U4,U5,U6 -All 5 snRNAs join the spliceosome to play crucial roles in splicing 14-12
14-12 snRNPs ✓Small nuclear RNAs (核内小RNA) coupled to proteins are abbreviated as snRNPs, small nuclear ribonuclear proteins ✓The snRNAs can be resolved on a gel: – U1, U2, U4, U5, U6 – All 5 snRNAs join the spliceosome to play crucial roles in splicing
Recognition of typical mammalian pre- mRNA intron by RNAs and protein U1 snRNP recognizes the 5'-splice site first,and then is replaced by U6 snRNP U2 snRNP recognizes the branchpoint,and then protein U2AF(U2-associated factor)recognizes the 3'-splice site U5 snRNP binds to the 5'-splice and 3'-splice site after initial recognization by ather U2AF AGGUAAGu -YNCURAC-Y NYAGgt 5'-splice site Branchpoint 3'-splice site 14-13
14-13 ✓ U1 snRNP recognizes the 5’-splice site first, and then is replaced by U6 snRNP ✓ U2 snRNP recognizes the branchpoint, and then protein U2AF (U2-associated factor) recognizes the 3’-splice site ✓ U5 snRNP binds to the 5’-splice and 3’-splice site after initial recognization by ather Recognition of typical mammalian premRNA intron by RNAs and protein