上海充更大 Shanghai Jiao Tong University Gram stain革兰氏染色 This staining method has long been used for classifying and identifying bacteria (For more detail:Lecture 3-1) Shanghai Jiao Tong University
Shanghai Jiao Tong University Shanghai Jiao Tong University Gram stain革兰氏染色 This staining method has long been used for classifying and identifying bacteria (For more detail: Lecture 3-1)
Step 1 Flood the heat-fixed smear with crystal violet for 1 min 结晶紫染1分钟 All cells purple Step 2 Add iodine solution for 3 min 碘液媒染3分钟 All cells remain purple Steps in the Gram stain procedure.革兰氏染色过程
Steps in the Gram stain procedure.革兰氏染色过程 结晶紫染1分钟 碘液媒染3分钟
Step 3 Decolorize with alcohol briefly -about 20 sec 酒精脱色20秒 Gram-positive cells are purple;gram- negative cells are colorless Step 4 Counterstain with safranin for 1-2 min 沙黄复染1-2分钟 Gram-positive(G+)cells are purple;gram-negative FLASH Gx (G)cells are pink to red
酒精脱色20秒 沙黄复染1-2分钟 FLASH
(⊙ ( Examples of Gram Staining
Examples of Gram Staining
国 Differences in cell wall structure are at the base of differences in the Gram-staining reaction.The G-cell wall is a multilayered structure and quite complex,whereas the G+cell wall consists of primarily a single type of molecule and is often much thicker. Gram-positive Gram-negative Peptidoglycan Peptidoglycan Membrane Membrane Periplasm Lipopolysaccharide and protein Cell walls of Bacteria FLASH
Differences in cell wall structure are at the base of differences in the Gram-staining reaction. The G- cell wall is a multilayered structure and quite complex, whereas the G+ cell wall consists of primarily a single type of molecule and is often much thicker. Cell walls of Bacteria FLASH