P-w丽履量-P-P Wild-type: T-antigen Mutant-type: T-antigen In nucleus in cytosol
Wild -type: T-antigen in nucleus Mutant -type: T-antigen in cytosol
Nuclear import and export Nuclear import receptors bind NLs and Nucleoporins The Ran gTPase drives directional transport through npc Ran- GDP The compartmentalization of Ran-GDP and Ran-GTP GDP Ran- GAP CYTOSOL NUCLEUS Ran-GEFI GDP GTP chromatin Ran- GTP
❖Nuclear import and export ➢ Nuclear import receptors bind NLS and Nucleoporins ➢ The Ran GTPase drives directional transport through NPC The compartmentalization of Ran-GDP and Ran-GTP
>A model for how GtP hydrolysis by ran provides directionality for nuclear transport nuclear import receptor nuclear export receptor Ran-GDP DISSOCIATION cargo delivered protein to cytosol th nuclear localization Ran-GDP signal E CYTOSOL NUCLEUS protein with Ran- GTP一@ nuclear export signal- cargo delivered Ran-GTP BINDING Qn to nucleus Ran-GTP
➢A model for how GTP hydrolysis by Ran provides directionality for nuclear transport
Nuclear export works like nuclear import but in reverse hnrnp proteins contain a nuclear-export signal (nes) Reference Cell92:327.1998
➢Nuclear export works like nuclear import, but in reverse hnRNP proteins contain a nuclear-export signal (NES) Reference: Cell 92: 327, 1998
2. Eukaryotes package DNA in Chromatin and chromosomes u Chromosomes exist in different states throughout the life of a cell Chromatin: (Interphase) Fibers. 10-30nm in diameter. Dispersed through the nucleus. DNA+ProteinS+RNA Chromosomes:(M phase) Cell division these fibers condense and fold into larger, compact structure
2. Eukaryotes package DNA in Chromatin and chromosomes ❑Chromosomes exist in different states throughout the life of a cell Chromatin: (Interphase) Fibers, 10-30nm in diameter, Dispersed through the nucleus. DNA+Proteins+RNA. Chromosomes: (M phase) Cell division, these fibers condense and fold into larger, compact structure