Gene cloning, expression and functional stud 基因克隆,表达及功能研究
Gene cloning, expression and functional study 基因克隆，表达及功能研究
vectors Cloning vectors:克隆载体 to clone a gene in a vector Expression vectors:表达载体 to express a gene from a vector Integration vectors:整合载体 to integrate a gene in a genome through a vector
vectors Cloning vectors: 克隆载体 to clone a gene in a vector Expression vectors: 表达载体 to express a gene from a vector Integration vectors: 整合载体 to integrate a gene in a genome through a vector
Cloning vectors 1 Plasmid vecters 2 Bacteriophage vectors 3 Cosmids BACs 4 Eukaryotic vectors
Cloning vectors 1 Plasmid vecters 2 Bacteriophage vectors 3 Cosmids & BACs 4 Eukaryotic vectors
Cloning vectors: allowing the exogenous dna to be inserted, stored and manipulated mainly at DNa level expression vectors: allowing the exogenous dna to be inserted, stored and expressed
Cloning vectors: allowing the exogenous DNA to be inserted, stored, and manipulated mainly at DNA level. expression vectors: allowing the exogenous DNA to be inserted, stored, and expressed
A plasmid vector for cloning 1. Contains an origin of replication, allowing for replication independent of host's genome. 2. Contains Selective markers: Selection of cells containing a plasmid twin antibiotic resistance blue-white screening 3. Contains a multiple cloning site(MCS 4. easy to be isolated from the host cell
1. Contains an origin of replication, allowing for replication independent of host’s genome. 2. Contains Selective markers: Selection of cells containing a plasmid twin antibiotic resistance blue-white screening 3. Contains a multiple cloning site (MCS) 4. Easy to be isolated from the host cell. A plasmid vector for cloning