生物信息字 普通高等教育 “十二五”规划教材 生物信息学 Bioinformatics 。。 第十二章：第二代测序技术 及其应用 Sequence 2.0
第十二章：第二代测序技术 及其应用 普通高等教育 “十二五”规划教材 生物信息学 Bioinformatics Sequence 2.0
Web 2.0 Coined by Darcy DiNucci in 1999. Resurface in 2003. 2004,O'Reilly Media and MediaLive hosted the first Web 2.0 conference. Examples of Web 2.0 include web-based communities, hosted services,web applications,social-networking sites,video-sharing sites,wikis,blogs,mashups and folksonomies.A Web 2.0 site allows its users to interact with other users or to change website content,in contrast to non-interactive websites where users are limited to the passive viewing of information that is provided to them. Ajax/Flash/Flex etc
Web 2.0 Coined by Darcy DiNucci in 1999. Resurface in 2003. 2004, O'Reilly Media and MediaLive hosted the first Web 2.0 conference. Examples of Web 2.0 include web-based communities, hosted services, web applications, social-networking sites, video-sharing sites, wikis, blogs, mashups and folksonomies. A Web 2.0 site allows its users to interact with other users or to change website content, in contrast to non-interactive websites where users are limited to the passive viewing of information that is provided to them. Ajax/Flash/Flex etc
Web 2.0 Meme Map Fllckr.del.iclo.us: PageRank. Blogs:Participation. BItTorrent Tagging. eBayre0pu的on, Not publishing Amazon revews:user Radical Decentr阳2aton not taxpnomy as contributor Gmall,Google Google AdSense: Maps and AIAX: customer self-servoe Wiklpedia: Rich User Experences enabling the long tall Radical Trust Strategic Positioning: The Wob as Platform User Posltioning: "An amtude,not You control your own data Trust your users a technology Core Competencles: Services,not packaged software Architecture of Particlpatlon Cost-effective scalablllty Small Pleces The Long Tail Remlxable data source and data transformations Loosely Joined Sottware abovo the lovel of a single device (web as components) Harnessing collective intelligence Data as the "intel Inside" Sofware that gets Rich User Experlence The perpetual beta better Play the more people use it Granular Addressabllty The Right to Remix Emergent:User of content Hackability behavor not Some rights reserved" predetermined
Next-generation DNA sequencing BAC-based sequencing,whole-genome sequencing (WGS) >1970s-2004,HGP Sanger/Maxam-Gilbert Next-generation DNA sequencing (NGS) >2004-now Second-generation DNA sequencing Deep-sequencing High-throughput DNA sequencing
Next-generation DNA sequencing BAC-based sequencing, whole-genome sequencing (WGS) ➢ 1970s-2004, HGP ➢ Sanger/Maxam-Gilbert Next-generation DNA sequencing (NGS) ➢ 2004-now ➢ Second-generation DNA sequencing ➢ Deep-sequencing ➢ High-throughput DNA sequencing
Second-generation DNA sequencing technologies Roche/454 FLX Pyrosequencer Illumina/Solexa Genome Analyzer Applied Biosystems SOLiDTM Sequencer Pacific Sequencer HeliScope Single Molecule Sequencer Cost per Cost per 1°error Feature generation Sequencing by synthesis megabase instrument Paired ends? modality Read-length 454 Emulsion PCR Polymerase(pyrosequencing) -$60 $500.000 Yes Indel 250bp Solexa Bridge PCR Polymerase (reversible terminators) -$2 $430.000 Yes Subst. 36bp SOLiD Emulsion PCR Ligase (octamers with two-base encoding) -$2 $591,000 Yes Subst. 35 bp Polonator Emulsion PCR Ligase(nonamers) -$1 $155.000 Yes Subst. 13bp HeliScope Single molecule Polymerase (asynchronous extensions) -$1 $1,350,000 Yes Del 30 bp
Second-generation DNA sequencing technologies Roche/454 FLX Pyrosequencer Illumina/Solexa Genome Analyzer Applied Biosystems SOLiDTM Sequencer Pacific Sequencer HeliScope Single Molecule Sequencer