BacteriaArchaeaEucaryaAnimalsGreennonsulfurSlimeEntamoebaebacteriaMoldsMitochondrionFungiMethanosarcinaGramPlantsExtremePurpleMethanobacteriumpositiveHalophilesbacteriaThermobacteriaCiliatesMethanoChloroplastproteuscocoueThemoPyrodictiumCOCCCyanobacteriaFlagellatesHavobacteria-TrichomonadsThermotogaAquifexMicrosporidiaDiplomonadsCommonAncestor(s)(Giardia)MarsSETG2008
Mars SETG 2008
BacteriaArchaeaEucaryaRuvkunetal.careerdownthetubes2008AnimalsGreennonsulfurSlimeEntamoebaebacteriaMoldsMitochondriorFungiMethanosarcinaGramPlantsExtremePurpleMethanobacteriumpositiveHalophilesbacteriaThermobacteriaMethano-CiliatesChloroplastproteuscoccusThemoPyrodictiumcOcCUCyanobacteriaFlagellatesFavobacteriaTrichomonadsThermotogaAquifexMicrosporidiaDiplomonadsCommonAncestor(s)(Giardia)
Ruvkun et al. career down the tubes 2008
Micrococcal nuclease reduces DNA contaminationbutnotPCRsensitivityMN106105104103102101100H.sal.genomesNoMNWithnoMN,DNA contamination comesupat1oogenomes
Micrococcal nuclease reduces DNA contamination but not PCR sensitivity MN – + + + + + + + H. sal. genomes 0 106 105 104 103 102 101 100 With no MN, DNA contamination comes up at 100 genomes No MN
Is the 16S ribosomal RNA gene the best target for life-detection by PCR?Are therebetteruniversal genes for life detection?approachiterative wholegenome sequence comparison across thetree of liferesults16S/23Sregionsaremostconserved,tRNAsnext,thenotherstuff
Is the 16S ribosomal RNA gene the best target for life-detection by PCR? Are there better universal genes for life detection? approach iterative whole genome sequence comparison across the tree of life results 16S/23S regions are most conserved, tRNAs next, then other stuff
Atum2bacteria二mitlochondrionrePaerRhodod顺Ecolarchaeayobimarine low tempEschenaaeaMjanePfur福ocyclusSyneMethar保O2QsaoooeThemococcusanobactenitHsalplasoferaxchGHalAnvcChilorobiumMethanospirillumMethLeptonemamarine Gp. 1 low tempClostridiumGp.1lowtemp.Gp.2iowtempBacillusPSL12BsubPSLHeliobacterium22Gp.3lowtemprobacter19SulfolobusArthr0dictium$66Root09O22IESsolDrad2AaeoSc8CeleZoa0.1 changes per siteCryptomonas404sooegGinaisoooePhysFuaoensarumTrypanosomaEuglenaeukaryaEncephalitozooVairimno
eukarya archaea bacteria Aaeo Drad Syne Ecol Atum Paer Bsub Pfur Mjan Ssol Hsal Cele Scer