CO2InorganiccompoundProtonElectronCarbonATPflowmotiveflowforce+so02SO42-NO3Biosynthesis(b)Chemolithotrophicmetabolism
PeriplasmCellmembraneFe3+CellwallFe2+Out(pH2)RusticyaninH+H+H+cytccytaNAD+12e1/2022H+H20In (pH 6)ADPCO2+ATPATPNADHCalvincycle
Eo (M)CoupleExamplesofreactions-0.50withH,asedonorCO2/glucose(-0.43)24e2H+/H2(-0.42)2e0.40CO,/methanol(-0.38)6e0.30NAD+/NADH(-0.32)2e(1)(1) H2 + fumarate?-→ succinate?-0.20CO,/acetate(-0.28)8eAG%=-86kJS/HS(-0.28)2e0.10SO2-/HS(-0.22)8e0.0Pyruvate/lactate(-0.19)2e-S.O62-/SO32-(+0.024)2e+0.10Fumarate/succinate(+0.03)2e+0.20(2)(2) H2 + NO → NO2 + H,OCytochromeboxreg(+0.035)1e+0.30AG= -163kJFe3+/Fe2+(+0.2)1e,(pH7)Ubiquinoneox/red(+0.11)2e+0.40Cytochrome Cox/red (+0.25)1e+0.50Cytochromeaox/red (+0.39)1eNO3-/NO(+0.42)2e+0.60+0.70WNO3-/%N（+0.74)5e(3) H, + 2 02 →H,0(3)+0.80-Fe3+/Fe2+(+0.76)1e,(pH2)AG%=-237kJ0/H0(+0.82)2e+0.90
515Fisoneuniversal16Sprimer5'GTGCCAGCAG CCGCGGTAAT3'UNIVERSALH.SAPIGGCAAGTCTG GTGCCAGCAG CCGCGGTAAT TCCAGCTCCA ATAGCGTATA650ANIMAL615X.LAEVGGCAAGTCTGGTGCCAGCAG CCGCGGTAAT TCCAGCTCCA ATAGCGTATAS.CERV601FUNGALGGCAAGTCTG GTGCCAGCAG CCGCGGTAAT TCCAGCTCCA ATAGCGTATA601P.MICAGGCAAGTCTGGTGCCAGCAG CCGCGGTAAT TCCAGCTCCA ATAGCGTATADINOFLAZ.MAYS605PLANTGGCAAGTCTG GTGCCAGCAG CCGCGGTAAT TCCAGCTCCA ATAGCGTATAGIARDIGCAAGGTCTGGTGCCAGCAG CCGCGGTAAT TCCAGCTCGG CGAGCGTCGC481PROTOZO516D.MOBIGGCAAGTCTG GTGTCAGCCG CCGCGGTAAT ACCAGCCCCG CGAGTGGTCGARCHAEA513S.SOLFGGCAAGTCTGGTGTCAGCCG CCGCGGTAAT ACCAGCTCCGCGAGTGGTCG513GGTAAGTCTG GTGTCAGCCG CCGCGGTAAT ACCAGCCCCG CGAGTGGTCAT.TENX500E0CY89GGCAAGTCGG GTGTCAGCCGCCGCGGTAAT ACCCGCTCCC CGAGTGGTGG20EOCY78GGCAAGGCTG GTGGCAGCCG CCGCGGTAAA ACCAGCTCCC CGAGGGGTTCGGCAAGTCTG GTGTCAGCCG CCGCGGTAAT ACCAGCCCCG CGAGCGGTCGPYRODIMPYRUSGGCAAGACCG CTGCCAGCCG CCGCGGTAAT AGCGGCGCCG CAAGTGGTGGT.CELRGGCAAGGCCG GTGGCAGCCG CCGCGGTAAT ACCGGCGGCC CGAGTGGTGG507ARGLB.GGCAAGGCCG GTGGCAGCCG CCGCGGTAAT ACCGGCGGCCCGAGTGGCGG492T.ACIDGGCAAGACGG GTGCCAGCCG CCGCGGTAAC ACCCGCAGCT CGAGTGGTGA486M.VANNGGCAAGTTCGGTGCCAGCAGCCGCGGTAAT ACCGACGGCCCGAGTGGTAG805MB.FORGGCAAGACCGACCGGCAGCTCAAGTGGTGGGTGCCAGCCGCCGCGGTAACMS.HUNGGCAAGACCGGTGCCAGCCGCCGCGGTAAT ACCGGCGGCTCGAGTGGTGG488M.SOEHGGCAAGACCG GTGCCAGCCG CCGCGGTAAC ACCGGCGGCT CGAGTGGTAA492H.VOLCGGCAAGACCG GTGCCAGCCG CCGCGGTAAT ACCGGCAGCT CAAGTGATGA机HC.MORGGCAAGACCG GTGCCAGCCG CCGCGGTAAT ACCGGCAGCT CGAGTGATAGH.HALOGGCAAGACCG GTGCCAGCCG CCGCGGTAAT ACCGGCAGTC CGAGTGATGGH.CUTIGGCAAGACCG GTGCCAGCCG CCGCGGTAAT ACCGGCAGTC CGAGTGATGGT.MTMAGGCTAACTACGTGCCAGCAG CCGCGGTAAT ACNTAGGGGG CAAGCGTTACBACTTM.ROSGGCTAACTACGTGCCAGCAG CCGCGGTAAG ACGTAGGGGG CGAGCGTTACCFX.AUGGCTAACTCT GTGCCAGCAG CCGCGGTAAGACAGAGGGGG CNAGCGTTGTAN.NIDGGCTAATTCC GTGCCAGCAG CCGCGGTAAT ACGGGAGAGGCAAGCGTTATBC.SUBGGCTAACTACGTGCCAGCAGCCGCGGTAAT ACGTAGGTGGCAAGCGTTTTE.COLIGGCTAACTCC GTGCCAGCAG CCGCGGTAAT ACGGAGGGTG CAAGCGTTAAAG.TUMGGCTAACTTC GTGCCAGCAG CCGCGGTAAT ACGAAGGGGG CTAGCGTTGT501Z.MCHLGGCTAACTCTGTGCCAGCAG CCGCGGTAAG ACAGAGGATG CAAGCGTTATCHLOROP
[0-7{0-10][0-559][0-837][0-180]V4[0-2][0-196524(0-146)V8[0-310]10-1571505[0-10][0-15][0-410.2[0-10]Reference sequenceand structure: Escherichie coli1J01695)t.celulerorgarNovembe2001[3:245] 5Numeerotsequences:73550-25V9Positons with a nuclectide in rrore than 95% of the soguences are[0,11] [0-179]shown in one ot feur categories.ACGU -98+%conservedacgu-90.9% conoerved - 80-90% conserved10-1-lossthan80%conservedOtherwise, the segions are represented by arcsDArclsbeisindicntetheurperandlowernumbercfmuciechcoskenownto axistwithin theassociabodvariableregion.10-411Blue tags indicate insertions relafive to the reference stquenteIserions tmat aopear are:(1) longih 1-4 in more than 10% ot soguences(2) orgth 5 or greater in at ieast one sequence10-560withformat:(Max.Length ofirserton:Poroentageof socswinary iongih ineertion)Citatonandreiatedintormationavailsbleathttp:/wwwrnaicmb.utexas.edu
V9 V8 V4 524 1505