Mass Spectrum 中p 分子量、分子式的确定 必1、从同位素相对峰强度计算 Example: The mass spectrum of an unknown compound has a molecular ion with a relative intensity of 43.27%, and an M+1 peak with a relative intensity of 3.81%.How many carbon atoms are there in the compound?
Mass Spectrum ❖——分子量、分子式的确定 ❖1、 从同位素相对峰强度计算 ❖Example: The mass spectrum of an unknown compound has a molecular ion with a relative intensity of 43.27%, and an M+1 peak with a relative intensity of 3.81%. How many carbon atoms are there in the compound?
Mass Spectrum *Solution:There are two naturally isotopes of carbon:98.89%12C and 1.11%13℃ M+1峰相对强度 3.81 碳原子数 8 0.011xM峰相对强度 0.011x43.27 同位数丰度表见教材(P30)
Mass Spectrum ❖Solution: There are two naturally isotopes of carbon: 98.89% 12C and 1.11% 13C ❖同位数丰度表见教材（P30） M+1 峰相对强度 3.81 碳原子数 = = = 8 0.011 x M 峰相对强度 0.011 x 43.27
Mass Spectrum 用 2、从高分辨质谱计算分子量 High-Resolution Mass Spectrometry can determine the exact molecular mass of a fragment with a precision of 0.0001amu
Mass Spectrum ❖2、 从高分辨质谱计算分子量 ❖High-Resolution Mass Spectrometry can determine the exact molecular mass of a fragment with a precision of 0.0001 amu