Pressure/suction alternately appli to mix medium F+(Strain A)- (Strain B) : Plate on Medium passes Plate on minimal medium across filter; bacterial minimal medil and incubate cells do not and incubate
F factor hromosome F- cell F cel Conjugation F+x F One strand of the f factor is nicked by an endonuclease This strand moves across the conjugation tube As the strand proceeds, complementary DNA synthesis occurs on both strands = Movement across conjugation F+ cell tube completed: Exconjugants DNA synthesis completed