IdentificationTriple sugar iron agar0.1% glucose1% lactose1% sucroseslantFerrous sulfatepH indicator: phenol redUninoculatedSHIHEZI UNIVERSITY
0.1% glucose 1% lactose 1% sucrose Ferrous sulfate pH indicator: phenol red Identification Triple sugar iron agar SHIHEZI UNIVERSITY
KIAA/AA/A+H2SKIA+H2STriple Sugar IronAgarPhoto by Karch MAlk/A/AA/AtgS A/A+H-SAlkAtgaeon
Triple Sugar Iron Agar
Identification Based On LactoseFermentation & MotilityTable162.Rapidpresumptiveidentificationofgram-negativeentericbacteriaLactoseFermented RapidlyLactoseFermented SlowlyLactose Not FermentedEscherichiacolimetllicsheenonwaShigellaecieomotilegamdiferentialmediamotilfatdextroseariznrovidenciawinnonviscous coloniesSalmonella species:motile;acid andEnterobacteraerogeneraisedcolonieusually gasfrom dextroseProteuspeciesswarmingoaganomeallicsheenoftenmotilemoreviscous growthurea rapidyhydrolyzed (smellofKlesillapneumonaveyviscouammonia)mucoid growth;nonmotilePseudomonasspecies(seeChapter17)ubigmbueeafuorescing; weetish smellE. coliH2S -motileLactose fermentedH2S .ShigellanonmotileLactose not fermentedH2S +motileSamonellaLactose not fermentedSHIHEZI UNIVERSITY
Identification Based On Lactose Fermentation & Motility E. coli motile Lactose fermented H2S - Shigella nonmotile Lactose not fermented H2S - Samonella motile Lactose not fermented H2S + SHIHEZI UNIVERSITY
Antigenic Structures O antigenLipopolysaccharide LipopolysacchrideO side chains (0) H antigenCapsule (K) FlagllaFlagella(H) K antigenCapsuleVi antigen In Salmonella TyphiCellenvelope(cytoplasmic membrane,peptidoglycan,outermembranthe capsular antigenis called Vi antigenFigureAntignicstructurefEnerobactaceae.SHIHEZI UNIVERSITY
Antigenic Structures O antigen Lipopolysacchride H antigen Flagella K antigen Capsule Vi antigen In Salmonella Typhi, the capsular antigen is called Vi antigen SHIHEZI UNIVERSITY
Antigenic StructuresEnterobacteriaceae are classified byMore than 150 different heatstable somatic O (LPSantigensMore than 100 heat-labile K (capsular) antigensMore than 50 flagellar HantigensEnterobacteriaceae strains are named as0157:H7 E coli 075:K100:H5 Salmonella Schottmilleri 05:Hb:2SHIHEZI UNIVERSITY
Antigenic Structures Enterobacteriaceae are classified by More than 150 different heatstable somatic O (LPS) antigens More than 100 heat-labile K (capsular) antigens More than 50 flagellar H antigens Enterobacteriaceae strains are named as E coli O75:K100:H5 O157:H7 Salmonella Schottmülleri O5:Hb:2 SHIHEZI UNIVERSITY