Laboratory DiagnosisFor Bacterial InfectionsSHIHEZI UNIVERSITY
Laboratory Diagnosis Laboratory Diagnosis For Bacterial Infections For Bacterial Infections SHIHEZI UNIVERSITY
OutlineLaboratory diagnosis for bacteriainfections Morphological -- microscopicMolecularSerologicalAnimalSHIHEZI UNIVERSITY
Outline Laboratory diagnosis for Laboratory diagnosis for bacterial bacterial infections infections Morphological Morphological - microscopic microscopic Molecular Molecular Serological Serological Animal Animal SHIHEZI UNIVERSITY
BiosaftySHIHEZI UNIVERSITY
Biosafty Biosafty SHIHEZI UNIVERSITY
Laboratory proceduresfor infectious disease in humansMorphologic identification of the agent in stains ofspecimens or sections oftissues (microscopy)Culture isolation and identification of the agentDetection of antigen from the agent by immunologicassay (latexagglutinationAec)orbyfluorecinlabeled (or peroxidase-labeled) antibody stainsDNA-DNA or DNA-RNA hybridization to detectpathogen-specific genes in patients specimens Demonstration of meaningful antibody or cell-mediatedimmune responses to an infectious agentSHIHEZIUNIVERSITY
Laboratory procedures for infectious disease in humans Morphologic identification Morphologic identification of the agent in stains of of the agent in stains of specimens or sections of tissues (microscopy) specimens or sections of tissues (microscopy) Culture isolation Culture isolation and identification of the agent. and identification of the agent. Detection of Detection of antigen antigen from the agent by immunologic from the agent by immunologic assay (latex agglutination, EIA, etc) or by assay (latex agglutination, EIA, etc) or by fluorescein fluorescein- labeled (or labeled (or peroxidase peroxidase-labeled) antibody stains. labeled) antibody stains. DNA DNA-DNA or DNA DNA or DNA-RNA RNA hybridization to detect hybridization to detect pathogen pathogen-specific genes in patients specific genes in patients’’specimens. specimens. Demonstration of meaningful Demonstration of meaningful antibody antibody or cell or cell-mediated mediated immune responses to an infectious agent. immune responses to an infectious agent. SHIHEZI UNIVERSITY
Assays Morphological assaysLightorelectronmicroscopiesIsolation and differentiation Serological assaysAntigen-antibody assays Molecular assaysMicroorganism's gene (DNA&RNASHIHEZIUNIVERSITY
Assays Morphological Morphological assays assays Light or electron Light or electron microscopies microscopies Isolation and differentiation Isolation and differentiation Serological Serological assays assays Antigen Antigen-antibody assays antibody assays Molecular Molecular assays assays Microorganism’’s gene (DNA & RNA) s gene (DNA & RNA) SHIHEZI UNIVERSITY