CHAPIN 小NBR三EDN 生物技在有神中的应周 Dept of Plant Breeding and Seed Engineering
CHAPTER 12 APPLICATION OF BIOTECHNOLOGY IN PLANT BREEDING 生物技术在育种中的应用 BY Niu Yingze （牛应泽） Dept. of Plant Breeding and Seed Engineering
8 1Application of Cell and Tissue Culture in Plant Breeding 细胞组织培养在植物育种的应用 PLANT TISSUE CULTURE is a blanket term for the in vitro cultivation of all plant parts under aseptic conditions. "plant tissue culture," is used as a blanket term for protoplast, cell, tissue and organ curture under aseptic conditions Tissue culture is a technique of growing plant tissues on synthetic medium under controlled and aseptic conditions
§1 Application of Cell and Tissue Culture in Plant Breeding 细胞组织培养在植物育种的应用 PLANT TISSUE CULTURE is a blanket term for the in vitro cultivation of all plant parts under aseptic conditions. Tissue culture is a technique of growing plant tissues on synthetic medium under controlled and aseptic conditions
1. Types of Explants in Plant Tissue Culture 植物组织培养中的外植体类型 · Plantlet Organs(Roots, hypocotyls, cotyledons, leaves, ovary, ovule, anther, pistils, etc.) Embryos(ncl. immature seeds) Tissue(Shoot tip Root tip, Meristem, callus, stem(nodal) segment) Cell(cell suspension) · Protoplast Microspore (Young pollens)
1. Types of Explants in Plant Tissue Culture 植物组织培养中的外植体类型 • Plantlet • Organs (Roots, hypocotyls, cotyledons, leaves, ovary, ovule, anther, pistils, etc.) • Embryos (Incl. immature seeds) • Tissue (Shoot tip, Root tip, Meristem, callus, • stem (nodal) segment) • Cell (Cell suspension) • Protoplast • Microspore (Young pollens)
Ovary culture
Ovary Culture
成热种面 力胚田 Immature Embryo culture
Immature Embryo Culture