20.2 Eukaryotic RNA polymerases consist of many subunits Size No Features 1 Related to bacterial subunit Br 20 binds dna has CTD =(YSPTSPS) least n=26: mouse n=52 1 Related to bacterial subunit B 10u binds nucleotides Figure 20.2 2 Related to bacterial subunit ox. Eukaryotic RNa <1 2 Common to all 3 polymerases polymerase II 25鬥 Common to all 3 polymerases has >10 subunits Common to all 3 polymerases 消当
Figure 20.2 Eukaryotic RNA polymerase II has >10 subunits. 20.2 Eukaryotic RNA polymerases consist of many subunits
20.3 Promoter elements are defined by mutations and footprinting Cotransfection is the simultaneous transfection of two markers 消当
Cotransfection is the simultaneous transfection of two markers. 20.3 Promoter elements are defined by mutations and footprinting
Upstream Promoter Transcribed region 20.3 Promoter 八A elements are defined NA by mutations and Delete and reconnect footprinting Transcription Figure 20.3 Promoter boundaries can be determined by making deletions that progressively RNA still made deletion does not enter promote remove more material from one side. When one deletion fails to Delete and reconnect prevent RNa synthesis but the next stops transcription. the boundary of the promoter must lie between them No RNA made deletion has entered promoter 消当 Upstream boundary of pro between ends of deletions
Figure 20.3 Promoter boundaries can be determined by making deletions that progressively remove more material from one side. When one deletion fails to prevent RNA synthesis but the next stops transcription, the boundary of the promoter must lie between them. 20.3 Promoter elements are defined by mutations and footprinting
Pol I prom oters have two sequence components 20, RNA CTCCGAGTCGNNNNNNTGGGC Startpoint polymerase I has a Upstream control element Core prom oter 170-160-150140-130-120-110 -605040 +10+20 bipartite promoter 人AAA The initiation complex assembles in three stages Figure 20.4 Transcription units UBF UBFI binds to G-C-nich sequence for RNa polymerase I have a cp r oro mhe ep stream ontrol Av element. UBFl binds to both SL SL1 binds cooperati ath UBF 1 regions. after which SLl can bind RNA polymerase I then binds to the core promoter. The 八 AVMVVN nature of the interaction between the factors bound at the 题 upstream controlelement and those at the core promoter is not VVALv known 消当
Figure 20.4 Transcription units for RNA polymerase I have a core promoter separated by ~70 bp from the upstream control element. UBF1 binds to both regions, after which SL1 can bind. RNA polymerase I then binds to the core promoter. The nature of the interaction between the factors bound at the upstream control element and those at the core promoter is not known. 20.4 RNA polymerase I has a bipartite promoter
20.5RNA polymerase Ill uses both downstream and upstream promoters Preinitiation complex in eukaryotic transcription describes the assembly of transcription factors at the promoter before rna polymerase binds 消当
Preinitiation complex in eukaryotic transcription describes the assembly of transcription factors at the promoter before RNA polymerase binds. 20.5 RNA polymerase III uses both downstream and upstream promoters