Nuclear hormone receptor superfamil H3N-B%&HCoo erb-A oncoprotein 铺 thyroid hormone 区匪 哥 H retinoic acid 黼铺铺辅 dihy droxy D3 圃 estradiol 偬”通 glucocortic。id 区圃睡睡andr。gen 铺铺辅pr。 gesterone 校铺排排 aldosterone DNA binding domain 区 圃 hormone binding domain Other receptor proteins in this extensive gene family interact with certain eicosanoids and other sterols
THIRD MILITARY MEDICAL UNIVERSITY COLLEGE OF BASIC MEDICAL SCIENCES
3、核受体与DNA的相互作用 A、DBD( DNA binding domain)的结构特点 66-88aa形成两个Ⅲ类锌指结构 B、HRE( hormone response element)的结构 C、DBD与HER的识别和结合
THIRD MILITARY MEDICAL UNIVERSITY COLLEGE OF BASIC MEDICAL SCIENCES 3、核受体与DNA的相互作用 A、DBD(DNA binding domain)的结构特点: 66-88aa形成两个II类锌指结构 B、HRE(hormone response element)的结构 C、DBD与HER的识别和结合
Zinc Finger Motifs in DNA Binding Domain Nuclear hormone Receptor Superfamily Cys LY5 D-box F Zn z c P-box amino acid residues that interact with Hormone Response element
THIRD MILITARY MEDICAL UNIVERSITY COLLEGE OF BASIC MEDICAL SCIENCES P-box D-box
Theoretical Biophysics Group Beckman institute University of Dlinois a Urbana-Champaign zinc finger motif dimer interface reading helix DNA half site(6 bp)
THIRD MILITARY MEDICAL UNIVERSITY COLLEGE OF BASIC MEDICAL SCIENCES
Hormone Response Elements Element DNA sequence TCTTGT GRE 5-AGAACAnnnTGTTCT-3 glucocorticoid ACAAGA specific amino acids in DNA binding domain include val, Ser, and Gly. ERE 5-AGGTCAnnnTGACCT-3 estrogen
THIRD MILITARY MEDICAL UNIVERSITY COLLEGE OF BASIC MEDICAL SCIENCES ACAAGA TCTTGT