三国大学脑 基因表达调控研究方法 Techniques of gene expression and requlation 生物化学与分子生物学教研室 连继勤 2015.9 Department of Biochemistry Molecular Biology e
Department of Biochemistry & Molecular Biology 1 Techniques of gene expression and regulation 基因表达调控研究方法 生物化学与分子生物学教研室 连继勤 2015.9
Genes can be regulated at many levels DNA RNA PROTEIN TRANSCRIPTION TRANSLATION he"“ Central Dogma Department of Biochemistry Molecular Biology e
Department of Biochemistry & Molecular Biology 2 Genes can be regulated at many levels DNA RNA PROTEIN TRANSCRIPTION TRANSLATION The “Central Dogma
Control of Gene Expression-Transcription Factors Transcription factors(TFS) are proteins that bind to the dna and help to control gene expression. We call the sequences to which they bind transcription factor binding sites(TFBSs), which are a type of CIS-regulatory sequence Department of Biochemistry Molecular Biology e
Department of Biochemistry & Molecular Biology 3 Control of Gene Expression—Transcription Factors Transcription factors (TFs) are proteins that bind to the DNA and help to control gene expression. We call the sequences to which they bind transcription factor binding sites (TFBSs), which are a type of cis-regulatory sequence
Control of Gene Expression Transcription factor binding sites are found within larger functional units of the dNa called cis-regulatory elements There are two main type of cis-regulatory elements: promoters and cis-regulatory modules(sometimes calledenhancers) cis-regulatory module( CRM) TFBS Transcription factor binding site(TFBS) TFBS activators DNA O coactivator neral transcription ors RNA polymerase TATA box coding region RNA transcript Department of Biochemistry Molecular Biology e
Department of Biochemistry & Molecular Biology 4 Control of Gene Expression Transcription factor binding sites are found within larger functional units of the DNA called cis-regulatory elements. There are two main type of cis-regulatory elements: promoters, and cis-regulatory modules (sometimes called “enhancers”). TFBS TFBS transcription factor binding site (TFBS) cis-regulatory module (CRM)
转录因子调控的基本方式 、通过与顺式调控元件相互作用影响转录活性 通过与其它转录因子或辅助蛋白相互作用影响转录活性 cis-regulatory module( CRM) TFBS Transcription factor binding site(TFBS) TFBS activators DNA O coactivator neral transcription ors RNA polymerase TATA box coding region RNA transcript Department of Biochemistry Molecular Biology e
Department of Biochemistry & Molecular Biology 5 转录因子调控的基本方式 一、通过与顺式调控元件相互作用影响转录活性 二、通过与其它转录因子或辅助蛋白相互作用影响转录活性 TFBS TFBS transcription factor binding site (TFBS) cis-regulatory module (CRM)