Integration Assumptions MEK-CDCI3 4 T and T2 values of all the spins are equal. 4 No spin decoupling being cha performed. ◆TheS/ N ratio is good No sloping baseline in the spectrum THMMR
THNMR YAN Integration Assumptions: T1 and T2 values of all the spins are equal. No spin decoupling being performed. The S/N ratio is good. No sloping baseline in the spectrum
Spin Systems Dictiona A Spin System is a group of spins that are connected by scalar(through-bond) spin-spin AB coupling J. For practical purposes, in biological macromolecules such J couplings can usually be observed between hydrogen atoms that are separated by three or less covalent bonds. For each amino acid residue the nonlabile protons AB2 constitute one or several spin systems ABC Note AMX 磁等价=化学等价 +对任一核J相等 THMMR
THNMR YAN Spin Systems Dictionary: A Spin System is a group of spins that are connected by scalar (through-bond) spin-spin coupling J. For practical purposes, in biological macromolecules such J couplings can usually be observed between hydrogen atoms that are separated by three or less covalent bonds. For each amino acid residue the nonlabile protons constitute one or several spin systems. AB AB2 ABC AMX Note: 磁等价 = 化学等价 +对任一核J相等
Spin Systems of amino acids H-N HN一C 甘氨酸(Gly) 丝氨酸(Ser AX AMX HaN-c-C HsN一C一 CHa o SH 半胱氨酸cys 天冬胺(Asn) AMX AMX THMMR
THNMR YAN Spin Systems of amino acids AX AMX AMX AMX