Pneumacoccustyp Infe cton of mice use dies 1.2 DNA is the no oth bateria genetic material Heat- kiled smooth ba cteria mouse in es rough bade ria Mixture of Heat-kiled Cs smo oth ba dera rough bacteria Dead mouse has lie smo oth bacteria Figure 1.2 The transforming Extrad DNA M principle Is DNA Add to Iwe rough bateria ■■ 消当 Tran sformation
Figure 1.2 The transforming principle is DNA. 1.2 DNA is the genetic material
Bacteria are infected with phages labeled with sZP()in DNA or with 3os(o) in protein 1.2 DNA is the genetic material Separate phage coats and infe cted bacteria Phage coats contain 80%of Infected bacteria contain 70%o label I solate progeny phage particle Progeny phage s have 30 % of 32p label and <1% of 35s label Figure 1.3 The genetic material of phage 12 IS DNA 消当
Figure 1.3 The genetic material of phage T2 is DNA. 1.2 DNA is the genetic material
Cells that lack Tk gene cannot produce thymidine kinase and die in absence of thymidine 1.2 DNA is the 器 genetic material 器 Dead cells Add TKTDNA 参 Live cells Figure 1. 4 Eukaryotic cells 像器 can acquire a new phenotype 器象 as the result of transfection 像 by added DNA 象 Colony of Tk t cell 消当 Some cells ta ke up k gene descendants of transfected cell pile up into a colony
Figure 1.4 Eukaryotic cells can acquire a new phenotype as the result of transfection by added DNA. 1.2 DNA is the genetic material
1.3 NA is a double helix Antiparallel strands of the double helix are organized in opposite orientation, so that the 5 end of one strand is aligned with the 3 end of the other strand Base pairing describes the specific(complementary Interactions of adenine with thymine or of cytosine with thymine in a DNA double helix(the former is replaced by adenine with uracil in double helical RNA) Complementary base pairs are defined by the pairing reactions In double helical nucleic acids(A with T in DNA or with U in RNA and C with G Supercoiling describes the coiling of a closed duplex DNA in space so that It crosses over its own axis 消当
Antiparallel strands of the double helix are organized in opposite orientation, so that the 5′ end of one strand is aligned with the 3′ end of the other strand. Base pairing describes the specific (complementary) interactions of adenine with thymine or of cytosine with thymine in a DNA double helix (the former is replaced by adenine with uracil in double helical RNA). Complementary base pairs are defined by the pairing reactions in double helical nucleic acids (A with T in DNA or with U in RNA, and C with G). Supercoiling describes the coiling of a closed duplex DNA in space so that it crosses over its own axis. 1.3 DNA is a double helix
1. 3 DNA is a double helix ucleotide subunit CH <py imidine base Fioure 5 CH2 A polynucleotide Purine base chain consists of a eries of 5C-3C ugar-phosphate links that form a backbone from which the bases Sugar-phosphate backbone protrude 消当
Figure 1.5 A polynucleotide chain consists of a series of 5￠-3￠ sugar-phosphate links that form a backbone from which the bases protrude 1.3 DNA is a double helix