微生物学与免疫学实验模块三微生物的形态观察、生理生化反应及鉴定细菌的革兰氏染色模块三
微生物学与免疫学实验 模块三 微生物的形态观察、生理生化反应及鉴定 之 模块三 细菌的革兰氏染色
主要内容常用染色方法的介绍细菌的革兰氏染色方法草兰氏染色方法的原理实验所需要的试剂及器械等操作步骤与注意事项染色结果观案实验目的与要求了解不同的细菌染色方法的原理掌握革兰氏染色方法的操作技术
实验目的与要求 了解不同的细菌染色方法的原理 掌握革兰氏染色方法的操作技术 常用染色方法的介绍 细菌的革兰氏染色方法 革兰氏染色方法的原理 实验所需要的试剂及器械等 操作步骤与注意事项 染色结果观察 主要内容
常用染色方法Negative staining(负染）Negative, indirect, or background staining isachieved by mixing bacteria with an acidicstain such as nigrosin, India ink,or eosin,andirstslidethen spreading out the mixture on a slide toform afilm.The above stains will not penetrateand/stain the bacterial cells due to repulsionbetween the negative charge of the stainsandthe negativelycharged bacterial wall.+++++
Negative staining（负染） Negative, indirect, or background staining is achieved by mixing bacteria with an acidic stain such as nigrosin, India ink, or eosin, and then spreading out the mixture on a slide to form a film. The above stains will not penetrate and stain the bacterial cells due to repulsion between the negative charge of the stains and the negatively charged bacterial wall. 常用染色方法
NegativelyStained Bacillus:Negatively Stained Cocci(A)NegetativeCell(B) Endospore
Negatively Stained Bacillus : (A) Vegetative Cell (B) Endospore Negatively Stained Cocci
常用染色方法Simplestains（简单染色）The use of a single stain or dye tocreatecontrastbetweenthebacteria andthe backgroundisDyerepelledDye aftrected拉referred fo as simple staining.IfsBasic dye (+1 Becterfium (-)Acidic dye(-/Bacterium (--)Call stainedCellclearchiefvalue lies in its simplicity and)Simple stain technique(o) Negative staln techniqueease of use. Simple staining isoffen employed wheninformationaboutcellshape,size,andarrangementisdesired
Simple stains（简单染色） The use of a single stain or dye to create contrast between the bacteria and the background is referred to as simple staining. Its chief value lies in its simplicity and ease of use. Simple staining is often employed when information about cell shape, size, and arrangement is desired. 常用染色方法