Department of Genomics Proteomics Department of Genetics Biochemistry RXR ras BRCA actin Wnt 11
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蛋白质组分析中的数据分析流程 Intact oell Cell lysis 2-D gel Mixture of image proteins 颜红 Protein LC profile 2-D gels Protein LC Extract Collect spot fraction dgeston Individuel p otein Peptde Differental Peptde LC profile label LC profle Trypsin Trypsin aigeston dgestion Mixture of peptides Electrospray ctrospray or MALDI Mass Mass spectrum Gas pnase fragmentation MS/MS otein spectrum Identification Comparisons 12
12 蛋白质组分析中的数据分析流程
Image Capturing 2-D Electrophoresis and Analysis Diversified sample preparation system Sam ple Preparation High throughput 2-D Electrophoresis system Well-rounded biological mass spectrum analysis system High throughput expression system High speed protein preparing system Site Cutting and Enzymolysis LC/MS/MS Data Searching Mass Spedtrum Analysis 13
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蛋白质数据库 Internet site Useful utilities Site address Protein Search databases with PMF http://prospector.ucsf.edu prospector and MS/MS data PROWL Also Provide theoretical data http://prowl.rockefeller.edu/PROWL/ and useful protocols prowl.html ExPASy PMF database searches, http://us.expasy.org theoretical tools and links to many other sites Mascot Search databases with PMF http://www.matrixscience.com and MS/MS data and sequence tags PepMAPPER Search databases with PMF http://wolf.bms.umist.ac.uk/mapper/ data MOWSE Search databases with PMF http://srs.hgmp.mrc.ac.uk/cgi- data bin/mowse PredictProtein Gives theoretical fragments http://www.ebi.ac.uk/-rost/predictpr server and data on a protein amino otein/ acid sequence 14
14 Internet site Useful utilities Site address Protein prospector Search databases with PMF and MS/MS data. http://prospector.ucsf.edu PROWL Also Provide theoretical data and useful protocols http://prowl.rockefeller.edu/PROWL/ prowl.html ExPASy PMF database searches, theoretical tools and links to many other sites http://us.expasy.org Mascot Search databases with PMF and MS/MS data and sequence tags http://www.matrixscience.com PepMAPPER Search databases with PMF data http://wolf.bms.umist.ac.uk/mapper/ MOWSE Search databases with PMF data http://srs.hgmp.mrc.ac.uk/cgibin/mowse PredictProtein server Gives theoretical fragments and data on a protein amino acid sequence http://www.ebi.ac.uk/~rost/predictpr otein/ 蛋白质数据库
2.蛋白质组学在拟南芥发育研究中的 应用 ()在拟茶种手葫发申的应师 A DS 0h 30h 48h 72h 96h 15
15 2. 蛋白质组学在拟南芥发育研究中的 应用 （1）在拟南芥种子萌发中的应用