Replication of eukaryotes Eukaryotes have multiple origins. The space between two adjacent origins is called the replicon,a functional unit of replication. 5 ori ori ori ori 31 5 3 3 3 5 replicon Consisting of all the DNA replicated from one origin
Eukaryotes have multiple origins. The space between two adjacent origins is called the replicon, a functional unit of replication. Replication of eukaryotes 5’ 3’ ori ori ori ori 5’ 3’ 5’ 5’ 3’ 3’ 5’ 5 ’ 3’ replicon 3’ Consisting of all the DNA replicated from one origin
Electron micrograph of replicating Drosophila DNA showing multiple"eyes"(bubbles)
Electron micrograph of replicating Drosophila DNA showing multiple “eyes”(bubbles)
Eukaryotic replicons are small and replicate more slowly than bacterial DNA. Organism Replicons Length Movement Bacterium 1 4200kb 50,000 bp/min Yeast 50 40 kb 3,600 bp/min Fruit fly 3,500 40kb 2,600 bp/min Toad 15,000 200kb 500 bp/min Mouse 25,000 150kb 2,200 bp/min Plant 35,000 300kb
Section2 Biosynthesis process of DNA
Section2 Biosynthesis process of DNA
S 2.Biosynthesis process of DNA DNA replication system Template:double stranded DNA Substrate:dNTP Primer:short RNA fragment with a free 3'-OH end Enzyme:DNA-dependent DNA polymerase(DDDP) Other enzyme and protein factor
§ 2. Biosynthesis process of DNA DNA replication system Template: double stranded DNA Substrate: dNTP Primer: short RNA fragment with a free 3´-OH end Enzyme : DNA-dependent DNA polymerase (DDDP) Other enzyme and protein factor