Components of ribosome Prokaryote Eukaryote riboso Small Large riboso Small Large me subunit subunit me subunit subunit 70S 30S 50S 80S 40S 60S 16S- 28S-rRNA 5S-rRNA 18S- rRNA 5S-rRNA rRNA 23S-rRNA rRNA 5.8S-rRNA protein rpS rpL rpS rpL 21kinds 36 kinds 33kinds 49 kinds
Prokaryote Eukaryote riboso me Small subunit Large subunit riboso me Small subunit Large subunit S 70S 30S 50S 80S 40S 60S rRNA 16SrRNA 5S-rRNA 23S-rRNA 18SrRNA 28S-rRNA 5S-rRNA 5.8S-rRNA protein rpS 21kinds rpL 36 kinds rpS 33kinds rpL 49 kinds Components of ribosome
rRNA rRNA is important component in ribosome and plays a key role in ribosome assembling and functioning 1.Maintaining structure of ribosome.Delete rRNA and ribosome will collapse. 2.Involved in binding to mRNA. 3.Key component in protein synthesis
rRNA rRNA is important component in ribosome and plays a key role in ribosome assembling and functioning. 1. Maintaining structure of ribosome. Delete rRNA and ribosome will collapse. 2. Involved in binding to mRNA. 3. Key component in protein synthesis
Illustration of protein synthesis in prokaryote Small subunit P site (Peptidyl site) mRNA D/donor site P位 A位 A site E位 tRNA (Aminoacyl site) Acceptor site AA Nascent E site peptide (Exit site) chain 000C06 Large subunit
Illustration of protein synthesis in prokaryote A site (Aminoacyl site) Acceptor site P site (Peptidyl site) D/donor site E site (Exit site) Small subunit AA Large subunit Nascent peptide chain
Function of ribosome in protein synthesis 1 Containing protein-conducting channel 2Containing binding sites of IF,EF and RF ③Containing three RNA binding sites,.(donor site,D位)； (peptidyl site,P位)；(acceptor site,aminoacyl site,A位) Peptidyl transferase activity ⑤GTPase activity
Function of ribosome in protein synthesis ① Containing protein-conducting channel ② Containing binding sites of IF, EF and RF ③ Containing three RNA binding sites， （ donor site, D 位); ( peptidyl site , P 位）; （ acceptor site, aminoacyl site , A 位） ④ Peptidyl transferase activity ⑤ GTPase activity
Section 2 The Process of Protein Biosynthesis
The Process of Protein Biosynthesis Section 2