It is feasible to determine at least 10,000 protein structures within the next five years No new discoveries or methods are needed for this task The average cost per structure is expected to decrease significantly from $200,000 per structure to perhaps less than $20,000 per structure 0/27/2005 Chaoqun Wu, Fudan University
10/27/2005 Chaoqun Wu, Fudan University Chaoqun Wu, Fudan University 6 It is feasible to determine at least 10,000 protein structures within the next five years. No new discoveries or methods are needed for this task. The average cost per structure is expected to decrease significantly from $200,000 per structure to perhaps less than $20,000 per structure
多 庭端静 Large-scale protein structure modeling A small sample of the 1, 100 comparative models calculated for the proteins in the yeast genome is displayed over an image of a yeast cel
10/27/2005 Chaoqun Wu, Fudan University Chaoqun Wu, Fudan University 7 Large-scale protein structure modeling. A small sample of the 1,100 comparative models calculated for the proteins in the yeast genome is displayed over an image of a yeast cell
Experimental structure determination 蛋白质的 体外翻译 蛋自质的生选择性蛋白质 物活性检测入分子的突变 0/27/2005 Chaoqun Wu, Fudan University
10/27/2005 Chaoqun Wu, Fudan University Chaoqun Wu, Fudan University 8 Experimental structure determination 蛋白质的生 物活性检测 选择性蛋白质 分子的突变 蛋白质的 体外翻译
1. Target selection(Grouping The targets for structure determination will be 1.Individual domains rather than multi-domain proteins(Chris Sander, Millenium Information 2. Easier to determine by X-ray crystallography or NMR spectroscopy than the more flexible multi domain proteins 3. Done by pairwise comparison of all protein sequences, followed by clustering into groups of domains 4. The representatives of the groups without structurally defined members 5. Members that share at least 30% sequence identity (30-seq families), rather than those that correspond to superfamiles or fold families 0/27/2005 Chaoqun Wu, Fudan University
10/27/2005 Chaoqun Wu, Fudan University Chaoqun Wu, Fudan University 9 1. Target selection (Grouping) The targets for structure determination will be 1. Individual domains rather than multi-domain proteins (Chris Sander, Millenium Information). 2. Easier to determine by X-ray crystallography or NMR spectroscopy than the more flexible multidomain proteins. 3. Done by pairwise comparison of all protein sequences, followed by clustering into groups of domains. 4. The representatives of the groups without structurally defined members. 5. Members that share at least 30% sequence identity (30-seq families), rather than those that correspond to superfamiles or fold families. The targets for structure determination will be 1. Individual domains rather than multi-domain proteins (Chris Sander, Millenium Information). 2. Easier to determine by X-ray crystallography or NMR spectroscopy than the more flexible multidomain proteins. 3. Done by pairwise comparison of all protein sequences, followed by clustering into groups of domains. 4. The representatives of the groups without structurally defined members. 5. Members that share at least 30% sequence identity (30-seq families), rather than those that correspond to superfamiles or fold families
A variety of different criteria likely will be used for this task; for example ■ size of the family, biological knowledge about the family, distribution of the members among various organIsms, the pharmaceutical relevance the likelihood of a successful structure determination and so forth 0/27/2005 Chaoqun Wu, Fudan University
10/27/2005 Chaoqun Wu, Fudan University Chaoqun Wu, Fudan University 10 A variety of different criteria likely will be used for this task; for example: size of the family, biological knowledge about the family, distribution of the members among various organisms, the pharmaceutical relevance, the likelihood of a successful structure determination, and so forth . A variety of different criteria likely will be used for this task; for example: size of the family, biological knowledge about the family, distribution of the members among various organisms, the pharmaceutical relevance, the likelihood of a successful structure determination, and so forth