上游充通大学 SHANGHAI JIAO TONG UNIVERSITY 乳液冷冻干燥 聚合物 去离子水 去离子水 ◆ e 乳液 液氮 冷冻于燥 ◆ 真空干燥 Whang K,et al.A novel method to fabricate bioabsorbable scaffolds Polymer 1995,36:837-42
乳液冷冻干燥 Whang K,et al.A novel method to fabricate bioabsorbable scaffolds .Polymer 1995,36:837-42 真空干燥 液氮 去离子水 乳液 聚合物 去离子水 冷冻干燥
上游充通大学 SHANGHAI JIAO TONG UNIVERSITY 三维支架制备技术 特料 孔径 制备技术 加工 孔隙平 要求 1% 结构 lμm 溶剂流诞盐沥滤流诞 溶解 30~300 ~90 球状孔径、盐粒会残留 挤出沥滤 模具 热塑性 50~500 <80 球状孔径、盐粒会残留 纤维粘令 编织 织物 20~100 <85 孔隙结构不规整 乳液冷冻干燥 流诞 溶解 20-200 >90 热诱导相分离 流诞 溶解 <100 <97 孔隙高度贯穿，微孔结构 超临界流体技术流诞 非品相 <50 10~30 非贯穿孔，微孔结构 三维打印 因体自 溶解 45~150 <60 100%贯穿孔（三角、五角、 由成型 蜂窝状)
制备技术 加工 材料 要求 孔径 /m 孔隙率 /% 结构 溶剂流涎盐沥滤 流涎 溶解 30~300 ~90 球状孔径、盐粒会残留 挤出沥滤 模具 热塑性 50~500 <80 球状孔径、盐粒会残留 纤维粘合 编织 织物 20~100 <85 孔隙结构不规整 乳液冷冻干燥 流涎 溶解 20~200 >90 热诱导相分离 流涎 溶解 <100 <97 孔隙高度贯穿，微孔结构 超临界流体技术 流涎 非晶相 <50 10~30 非贯穿孔，微孔结构 三维打印 固体自 由成型 溶解 45~150 <60 100%贯穿孔（三角、五角、 蜂窝状） 三维支架制备技术
上游充通大学 SHANGHAI JIAO TONG UNIVERSITY World market of cell therapy 35,000 Cord blood and cell banking ■ Cancer General,Gl,gynecology,other 30,000- Ophthalmology Organ replacement/transplant Bone Cartllage Dental/oral 25,000- Skin/integumentary Urology Orthopedics,musculoskeletal,spine 20,000- Neurology 9 Cardiology BMSCs 15,000- 10,000- Adipocytes HP-supportive stroma 5,000 Critical Reviews in Oral Biology and Medicine,1999,10,165-81 2009 2010 2011201220122014°2015201620172018* Year B.Schachter,Nature Biotech.2014,32,736-741
Critical Reviews in Oral Biology and Medicine, 1999, 10, 165-81 World market of cell therapy B. Schachter, Nature Biotech. 2014, 32, 736-741
上游充通大学 SHANGHAI JIAO TONG UNIVERSITY 卵母细邂 干细胞治疗 发育 桑客胚 滋养层 亮胚：形成64-200 分化为不同的细胞 个细胞阶段 心肌细烟 内细胞群 体外增殖 病人 移植 肝细胞 神经细胞 细胞移植可治疗如下疾病：糖尿病、阿尔兹海默病 多潜能胚船干潮胞 帕金森、脊髓损伤、朝肉菱缩硬化建、悠崔、血管 疾病、风湿性关节炎等 陕腺细园 品道细圈
上游充通大学 SHANGHAI JIAO TONG UNIVERSITY Features uncleared: 2 marrow .The relationship between the implanted cells Bone matrix and the autologous repair cells raised in the Skeletal stem call defect area E viwo expangion 4 .Failure of autologous bone marrow transplantation is caused by the low tricalcium phosphate particles 888 concentration of viable stem cells [1] In vivo transplantation .In contrast,cases that only a very small 5 into segmental defect amount of implanted stem cells remains viable results in successful bone Materials Science and Engineering:C 2015,48,556-65 Nature,2001,414,118-121 transplantation [2] .Whether the implanted stem cells are actively integrated into or transformed into bone tissue,or act as a medium to release [1]Journal of bone and joint surgery 2005;87:896-902 [2]Nature reviews Endocrinology.2015;11:140-50;PNAS 2010;107:3305-10. related factors to promote endogenous cellular repair of bone tissue,or to collect autologous repair cells in the defect area
Materials Science and Engineering: C 2015, 48, 556-65 Nature, 2001, 414, 118-121 Features uncleared: •The relationship between the implanted cells and the autologous repair cells raised in the defect area •Failure of autologous bone marrow transplantation is caused by the low concentration of viable stem cells [1] •In contrast, cases that only a very small amount of implanted stem cells remains viable results in successful bone transplantation [2] •Whether the implanted stem cells are actively integrated into or transformed into bone tissue, or act as a medium to release related factors to promote endogenous cellular repair of bone tissue, or to collect autologous repair cells in the defect area [1] Journal of bone and joint surgery 2005;87:896-902 [2] Nature reviews Endocrinology. 2015;11:140-50; PNAS 2010;107:3305-10